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PMID:20394854
| Citation |
Capalbo, G, Dittmann, K, Weiss, C, Reichert, S, Hausmann, E, Rödel, C and Rödel, F (2010) Radiation-induced survivin nuclear accumulation is linked to DNA damage repair. Int. J. Radiat. Oncol. Biol. Phys. 77:226-34 |
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| Abstract |
Increased expression of survivin has been identified as a negative prognostic marker in a variety of human cancers. We have previously shown that survivin is a radiation-resistance factor and that the therapeutic effect of survivin knock-down might result from an impaired DNA repair capacity. In this study, we aimed to elucidate an interrelationship between survivin's cellular localization and DNA double-strand break repair. |
| Links |
PubMed Online version:10.1016/j.ijrobp.2009.12.001 |
| Keywords |
Cell Nucleus/metabolism; Cytoplasm/metabolism; DNA Breaks, Double-Stranded; DNA Damage; DNA Repair/physiology; DNA Repair/radiation effects; DNA-Activated Protein Kinase/metabolism; Histones/metabolism; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Nuclear Proteins/metabolism; Phosphorylation; RNA, Small Interfering; Trans-Activators/metabolism; Tumor Cells, Cultured/radiation effects |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006302: double-strand break repair |
ECO:0000315: |
P |
Figure 3a. siRNA-Survivin knock down is shown to increase gamma-H2AX which means "survivin attenuation impairs double strand DNA repair capacities" (page 233, left column, bottom of 2nd paragraph). This is IMP because the use of siRNA to change the phenotype. It is not annotated further because this paper does not show evidence for a mechanism. |
complete | ||||
| GO:0031954: positive regulation of protein autophosphorylation |
ECO:0000315: |
P |
Figure 4b row 2 (DNA-PKcs S2056) and row 4 (survivin). When survivin is knocked down by siRNA (column 1 vs. 2) the phosphorylation (which DNA-PKcs autophosphorylates) of S2056 of DNA-PKcs is inhibited. "DNA-PKcs (auto)phosphorylation at Serine 2056 and observed an impaired detection of phosphoS2056 DNA-PKcs." (page 233, right column, first paragraph) It is IMP because of the use of siRNA |
complete | ||||
| GO:0010800: positive regulation of peptidyl-threonine phosphorylation |
ECO:0000315: |
P |
Figure 4b row 1 (DNA-PKcs T2609). In this figure, "decreased T2609 phosphoryltion of the DNA-PKcs moecule" was observed when survivin was knocked down. (page 229, left column, first paagraph) In the presence of survivin the T2609 is phosphorylated meaning the survivin is involved in the positive regulation of this. This is an IMP because si-RNA is used to change the phenotype. |
complete | ||||
See also
References
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