PMID:20195537

Citation	Pavankumar, TL, Sinha, AK and Ray, MK (2010) All three subunits of RecBCD enzyme are essential for DNA repair and low-temperature growth in the Antarctic Pseudomonas syringae Lz4W. PLoS ONE 5:e9412
Abstract	The recD mutants of the Antarctic Pseudomonas syringae Lz4W are sensitive to DNA-damaging agents and fail to grow at 4 degrees C. Generally, RecD associates with two other proteins (RecB and RecC) to produce RecBCD enzyme, which is involved in homologous recombination and DNA repair in many bacteria, including Escherichia coli. However, RecD is not essential for DNA repair, nor does its deletion cause any growth defects in E. coli. Hence, the assessment of the P. syringae RecBCD pathway was imperative.
Links	PubMed PMC2828478 Online version:10.1371/journal.pone.0009412
Keywords	Antarctic Regions; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Blotting, Western; Cell Division/drug effects; Cell Division/genetics; Cell Division/radiation effects; Cold Temperature; DNA Repair; Exodeoxyribonuclease V/genetics; Exodeoxyribonuclease V/metabolism; Genetic Complementation Test; Microbial Viability/drug effects; Microbial Viability/genetics; Microbial Viability/radiation effects; Mitomycin/pharmacology; Mutation; Nucleic Acid Synthesis Inhibitors/pharmacology; Operon/genetics; Protein Subunits/genetics; Protein Subunits/metabolism; Pseudomonas syringae/enzymology; Pseudomonas syringae/genetics; Pseudomonas syringae/growth & development; Recombination, Genetic; Ultraviolet Rays
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECODH:B1XDM6		GO:0006281: DNA repair	IMP: Inferred from Mutant Phenotype:	P	Figure 6 shows DNA repairing proficiency of RecBK28QCD, RecBD1118A, and RecBCDK229Q enzymes of P. syringae.	complete CACAO 4917

References

See Help:References for how to manage references in GONUTS.