PMID:20133733

Citation	Ehrentraut, S, Weber, JM, Dybowski, JN, Hoffmann, D and Ehrenhofer-Murray, AE (2010) Rpd3-dependent boundary formation at telomeres by removal of Sir2 substrate. Proc. Natl. Acad. Sci. U.S.A. 107:5522-7
Abstract	Boundaries between euchromatic and heterochromatic regions until now have been associated with chromatin-opening activities. Here, we identified an unexpected role for histone deacetylation in this process. Significantly, the histone deacetylase (HDAC) Rpd3 was necessary for boundary formation in Saccharomyces cerevisiae. rpd3Delta led to silent information regulator (SIR) spreading and repression of subtelomeric genes. In the absence of a known boundary factor, the histone acetyltransferase complex SAS-I, rpd3Delta caused inappropriate SIR spreading that was lethal to yeast cells. Notably, Rpd3 was capable of creating a boundary when targeted to heterochromatin. Our data suggest a mechanism for boundary formation whereby histone deacetylation by Rpd3 removes the substrate for the HDAC Sir2, so that Sir2 no longer can produce O-acetyl-ADP ribose (OAADPR) by consumption of NAD(+) in the deacetylation reaction. In essence, OAADPR therefore is unavailable for binding to Sir3, preventing SIR propagation.
Links	PubMed PMC2851772 Online version:10.1073/pnas.0909169107
Keywords	Acetylation; Euchromatin/genetics; Euchromatin/metabolism; Gene Silencing; Genes, Fungal; Heterochromatin/genetics; Heterochromatin/metabolism; Histone Acetyltransferases/genetics; Histone Acetyltransferases/metabolism; Histone Deacetylases/genetics; Histone Deacetylases/metabolism; Histones/chemistry; Histones/metabolism; Models, Molecular; Protein Structure, Tertiary; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Silent Information Regulator Proteins, Saccharomyces cerevisiae/chemistry; Silent Information Regulator Proteins, Saccharomyces cerevisiae/genetics; Silent Information Regulator Proteins, Saccharomyces cerevisiae/metabolism; Sirtuin 2/genetics; Sirtuin 2/metabolism; Substrate Specificity; Telomere/genetics; Telomere/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:RPD3	involved_in	GO:0031939: negative regulation of chromatin silencing at telomere	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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