PMID:20133583

Citation	Vasale, JJ, Gu, W, Thivierge, C, Batista, PJ, Claycomb, JM, Youngman, EM, Duchaine, TF, Mello, CC and Conte, D Jr (2010) Sequential rounds of RNA-dependent RNA transcription drive endogenous small-RNA biogenesis in the ERGO-1/Argonaute pathway. Proc. Natl. Acad. Sci. U.S.A. 107:3582-7
Abstract	Argonaute (AGO) proteins interact with distinct classes of small RNAs to direct multiple regulatory outcomes. In many organisms, including plants, fungi, and nematodes, cellular RNA-dependent RNA polymerases (RdRPs) use AGO targets as templates for amplification of silencing signals. Here, we show that distinct RdRPs function sequentially to produce small RNAs that target endogenous loci in Caenorhabditis elegans. We show that DCR-1, the RdRP RRF-3, and the dsRNA-binding protein RDE-4 are required for the biogenesis of 26-nt small RNAs with a 5' guanine (26G-RNAs) and that 26G-RNAs engage the Piwi-clade AGO, ERGO-1. Our findings support a model in which targeting by ERGO-1 recruits a second RdRP (RRF-1 or EGO-1), which in turn transcribes 22G-RNAs that interact with worm-specific AGOs (WAGOs) to direct gene silencing. ERGO-1 targets exhibit a nonrandom distribution in the genome and appear to include many gene duplications, suggesting that this pathway may control overexpression resulting from gene expansion.
Links	PubMed PMC2840456 Online version:10.1073/pnas.0911908107
Keywords	Animals; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Endoribonucleases/metabolism; Gene Silencing; RNA Interference; RNA Replicase/metabolism; RNA, Small Interfering/biosynthesis; RNA, Small Interfering/genetics; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Ribonuclease III; Transcription, Genetic
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CAEEL:DCR1		GO:0030422: production of siRNA involved in RNA interference	ECO:0000315:		P		Figure 4	complete CACAO 7438
CAEEL:DCR1	involved_in	GO:0030422: production of siRNA involved in RNA interference	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.