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PMID:20038682
| Citation |
Lechtreck, KF, Johnson, EC, Sakai, T, Cochran, D, Ballif, BA, Rush, J, Pazour, GJ, Ikebe, M and Witman, GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J. Cell Biol. 187:1117-32 |
|---|---|
| Abstract |
In humans, seven evolutionarily conserved genes that cause the cilia-related disorder Bardet-Biedl syndrome (BBS) encode proteins that form a complex termed the BBSome. The function of the BBSome in the cilium is not well understood. We purified a BBSome-like complex from Chlamydomonas reinhardtii flagella and found that it contains at least BBS1, -4, -5, -7, and -8 and undergoes intraflagellar transport (IFT) in association with a subset of IFT particles. C. reinhardtii insertional mutants defective in BBS1, -4, and -7 assemble motile, full-length flagella but lack the ability to phototax. In the bbs4 mutant, the assembly and transport of IFT particles are unaffected, but the flagella abnormally accumulate several signaling proteins that may disrupt phototaxis. We conclude that the BBSome is carried by IFT but is an adapter rather than an integral component of the IFT machinery. C. reinhardtii BBS4 may be required for the export of signaling proteins from the flagellum via IFT. |
| Links |
PubMed PMC2806276 Online version:10.1083/jcb.200909183 |
| Keywords |
Algal Proteins/genetics; Algal Proteins/metabolism; Algal Proteins/physiology; Axoneme/genetics; Axoneme/physiology; Chlamydomonas reinhardtii/genetics; Chlamydomonas reinhardtii/metabolism; Flagella/metabolism; Protein Transport/physiology; Signal Transduction |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0046957: negative phototaxis |
ECO:0000315: |
P |
Fig. 2A Transformation of mutant strain with BBS4 gene restored strong negative phototaxis characteristic of wt. |
complete | ||||
| GO:0043623: cellular protein complex assembly |
ECO:0000315: |
P |
Fig. 4 |
complete | ||||
| GO:0034464: BBSome |
ECO:0000314: |
C |
Fig. 4B |
complete | ||||
| GO:0005515: protein binding |
ECO:0000021: |
UniProtKB:A8HMT4
|
F |
Fig 4C |
complete | |||
| GO:0005515: protein binding |
ECO:0000021: |
UniProtKB:A8JEA1
|
F |
Fig 4C |
complete | |||
| GO:0005515: protein binding |
ECO:0000021: |
UniProtKB:A8JF63
|
F |
Fig 4C |
complete | |||
| GO:0005515: protein binding |
ECO:0000021: |
UniProtKB:A8HWI3
|
F |
Fig 4C. Note that there are 2 slightly different, unreviewed records for the binding partner - UniProtKB:A8HWI3 & UniProtKB:Q6PSU7. |
complete | |||
| GO:0005515: protein binding |
ECO:0000021: |
UniProtKB:A8IYF1_CHLRE
|
F |
Fig 4 |
complete | |||
| GO:0034464: BBSome |
ECO:0000314: |
C |
Fig 4 B and C |
complete | ||||
| GO:0005515: protein binding |
ECO:0000021: |
UniProtKB:A8IYF1_CHLRE
|
F |
Fig4 |
complete | |||
| GO:0034464: BBSome |
ECO:0000314: |
C |
Fig4 B and C |
complete | ||||
See also
References
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