PMID:19264778

Citation	Everett, RD, Parsy, ML and Orr, A (2009) Analysis of the functions of herpes simplex virus type 1 regulatory protein ICP0 that are critical for lytic infection and derepression of quiescent viral genomes. J. Virol. 83:4963-77
Abstract	Herpes simplex virus type 1 (HSV-1) immediate-early regulatory protein ICP0 is important for stimulating the initiation of the lytic cycle and efficient reactivation of latent or quiescent infection. Extensive investigation has suggested several potential functions for ICP0, including interference in the interferon response, disruption of functions connected with PML nuclear bodies (ND10), and inhibition of cellular histone deacetylase (HDAC) activity through an interaction with the HDAC-1 binding partner CoREST. Analysis of the significance of these potential functions and whether they are direct or indirect effects of ICP0 is complicated because HSV-1 mutants expressing mutant forms of ICP0 infect cells with widely differing efficiencies. On the other hand, transfection approaches for ICP0 expression do not allow studies of whole cell populations because of their limited efficiency. To overcome these problems, we have established a cell line in which ICP0 expression can be induced at levels pertaining during the early stages of HSV-1 infection in virtually all cells in the culture. Such cells enable 100% complementation of ICP0-null mutant HSV-1. Using cells expressing the wild type and a variety of mutant forms of ICP0, we have used this system to analyze the role of defined domains of the protein in stimulating lytic infection and derepression from quiescence. Activity in these core functions correlated well the ability of ICP0 to disrupt ND10 and inhibit the recruitment of ND10 proteins to sites closely associated with viral genomes at the onset of infection, whereas the CoREST binding region was neither sufficient nor necessary for ICP0 function in lytic and reactivating infections.
Links	PubMed PMC2682082 Online version:10.1128/JVI.02593-08
Keywords	Cell Line; Gene Expression Regulation, Viral; Genetic Complementation Test; Genome, Viral; Herpes Simplex/virology; Herpesvirus 1, Human/genetics; Herpesvirus 1, Human/metabolism; Herpesvirus 1, Human/pathogenicity; Humans; Immediate-Early Proteins/genetics; Immediate-Early Proteins/metabolism; Mutation; Nuclear Proteins/metabolism; Plasmids; Protein Interaction Domains and Motifs; Transduction, Genetic; Ubiquitin-Protein Ligases/genetics; Ubiquitin-Protein Ligases/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HHV11:ICP0		GO:0019046: release from viral latency	ECO:0000315:		P		Table 1 shows plaque formation (indicating cell lysis) before and after induction on ICP0 production. Table 10 also shows a summary of the data.	complete CACAO 5194

References

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