PMID:19169252

Citation	Sidiropoulou, K, Lu, FM, Fowler, MA, Xiao, R, Phillips, C, Ozkan, ED, Zhu, MX, White, FJ and Cooper, DC (2009) Dopamine modulates an mGluR5-mediated depolarization underlying prefrontal persistent activity. Nat. Neurosci. 12:190-9
Abstract	The intrinsic properties of neurons that enable them to maintain depolarized, persistently activated states in the absence of sustained input are poorly understood. In short-term memory tasks, individual prefrontal cortical (PFC) neurons can maintain persistent action potential output during delay periods between informative cues and behavioral responses. Dopamine and drugs of abuse alter PFC function and working memory, possibly by modulating intrinsic neuronal properties. Here we used patch-clamp recording of layer 5 PFC pyramidal neurons to identify a postsynaptic depolarization that was evoked by action potential bursts and mediated by metabotropic glutamate receptor 5 (mGluR5). This depolarization occurred in the absence of recurrent synaptic activity and was reduced by a dopamine D1 receptor (D1R) protein kinase A pathway. After behavioral sensitization to cocaine, the depolarization was substantially diminished and D1R modulation was lost. We propose that burst-evoked intrinsic depolarization is a form of short-term cellular memory that is modulated by dopamine and cocaine experience.
Links	PubMed PMC2727588 Online version:10.1038/nn.2245
Keywords	Action Potentials/drug effects; Action Potentials/physiology; Animals; Calcium/metabolism; Cocaine/pharmacology; Cyclic AMP-Dependent Protein Kinases/metabolism; Dopamine/metabolism; Dopamine Uptake Inhibitors/pharmacology; Male; Mice; Mice, Knockout; Neuronal Plasticity/drug effects; Neuronal Plasticity/physiology; Organ Culture Techniques; Patch-Clamp Techniques; Prefrontal Cortex/cytology; Prefrontal Cortex/physiology; Pyramidal Cells/physiology; Rats; Receptor, Metabotropic Glutamate 5; Receptors, Dopamine D1/metabolism; Receptors, Metabotropic Glutamate/physiology; Synapses/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:GRM5	enables	GO:0099530: G protein-coupled receptor activity involved in regulation of postsynaptic membrane potential	ECO:0000315: mutant phenotype evidence used in manual assertion		F	occurs_in:(GO:0060076) occurs_in:(CL:0000598) occurs_in:(UBERON:0000451)	Seeded From UniProt	complete

References

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