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PMID:19017647
| Citation |
Yu, Q, Kuzmiak, H, Zou, Y, Olsen, L, Defossez, PA and Bi, X (2009) Saccharomyces cerevisiae linker histone Hho1p functionally interacts with core histone H4 and negatively regulates the establishment of transcriptionally silent chromatin. J. Biol. Chem. 284:740-50 |
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| Abstract |
Saccharomyces cerevisiae linker histone Hho1p is not essential for cell viability, and very little is known about its function in vivo. We show that deletion of HHO1 (hho1Delta) suppresses the defect in transcriptional silencing caused by a mutation in the globular domain of histone H4. hho1Delta also suppresses the reduction in HML silencing by the deletion of SIR1 that is involved in the establishment of silent chromatin at HML. We further show that hho1Delta suppresses changes in silent chromatin structure caused by the histone H4 mutation and sir1Delta. These results suggest that HHO1 plays a negative role in transcriptionally silent chromatin. We also provide evidence that Hho1p hinders the de novo establishment of silent chromatin but does not affect the stability of preexistent silent chromatin. Unlike canonical linker histones in higher eukaryotes that have a single conserved globular domain, Hho1p possesses two globular domains. We show that the carboxyl-terminal globular domain of Hho1p is dispensable for its function, suggesting that the mode of Hho1p action is similar to that of canonical linker histones. |
| Links |
PubMed PMC2613606 Online version:10.1074/jbc.M806274200 |
| Keywords |
Chromatin/genetics; Gene Deletion; Gene Silencing; Histones/genetics; Histones/metabolism; Mutation/genetics; Protein Binding; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Silent Information Regulator Proteins, Saccharomyces cerevisiae/genetics; Silent Information Regulator Proteins, Saccharomyces cerevisiae/metabolism; Transcription, Genetic/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0031936: negative regulation of chromatin silencing |
ECO:0000316: genetic interaction evidence used in manual assertion |
SGD:S000001809 |
P |
Seeded From UniProt |
complete | ||
See also
References
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