GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:19008911
| Citation |
Okutsu, R, Rai, T, Kikuchi, A, Ohno, M, Uchida, K, Sasaki, S and Uchida, S (2008) AKAP220 colocalizes with AQP2 in the inner medullary collecting ducts. Kidney Int. 74:1429-33 |
|---|---|
| Abstract |
During dehydration, protein kinase A phosphorylates aquaporin 2 (AQP2) at serine 256 and this is essential for apical membrane sorting of AQP2 in the collecting ducts. A-kinase anchoring proteins (AKAPs) bind protein kinase A and protein phosphatases conferring substrate specificity to these enzymes and localize them to the appropriate intracellular compartment. We found that AKAP220 bound to AQP2 in a yeast two-hybrid screen. Further, it was highly localized to the papilla compared to other regions of the kidney. Using double immunofluorescence and immunoelectron microscopy we found that AKAP220 co-localized with AQP2 in the cytosol of the inner medullary collecting ducts. Forskolin-mediated phosphorylation of AQP2, transiently expressed in COS cells, was increased by AKAP220 co-expression. Our results suggest that AKAP220 may be involved in the phosphorylation of AQP2 by recruiting protein kinase A. |
| Links |
PubMed Online version:10.1038/ki.2008.402 |
| Keywords |
A Kinase Anchor Proteins/metabolism; Animals; Aquaporin 2/metabolism; Cyclic AMP-Dependent Protein Kinases/metabolism; Cytosol/chemistry; Kidney Medulla/chemistry; Kidney Tubules, Collecting/chemistry; Phosphorylation; Protein Binding; Rats |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0005737: cytoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
