PMID:18978066

Citation	Verhamme, DT, Murray, EJ and Stanley-Wall, NR (2009) DegU and Spo0A jointly control transcription of two loci required for complex colony development by Bacillus subtilis. J. Bacteriol. 191:100-8
Abstract	Biofilm formation is an example of a multicellular process which depends on cooperative behavior and differentiation within a bacterial population. Our findings indicate that there is a complex feedback loop that maintains the stoichiometry of the extracellular matrix and other proteins required for complex colony development by Bacillus subtilis. Analysis of the transcriptional regulation of two DegU-activated genes that are required for complex colony development by B. subtilis revealed additional involvement of global regulators that are central to controlling biofilm formation. Activation of transcription from both the yvcA and yuaB promoters requires DegU approximately phosphate, but transcription is inhibited by direct AbrB binding to the promoter regions. Inhibition of transcription by AbrB is relieved when Spo0A approximately phosphate is generated due to its known role in inhibiting abrB expression. Deletion of SinR, a key coordinator of motility and biofilm formation, enhanced transcription from both loci; however, no evidence of a direct interaction with SinR for either the yvcA or yuaB promoter regions was observed. The enhanced transcription in the sinR mutant background was subsequently demonstrated to be dependent on biosynthesis of the polysaccharide component that forms the major constituent of the B. subtilis biofilm matrix. Together, these findings indicate that a genetic network dependent on activation of both DegU and Spo0A controls complex colony development by B. subtilis.
Links	PubMed PMC2612447 Online version:10.1128/JB.01236-08
Keywords	Anti-Bacterial Agents/pharmacology; Bacillus subtilis/classification; Bacillus subtilis/drug effects; Bacillus subtilis/genetics; Bacillus subtilis/growth & development; Bacterial Proteins/metabolism; Biofilms; DNA Primers; Escherichia coli/drug effects; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Genotype; Operon; Plasmids; Polysaccharides, Bacterial/metabolism; Promoter Regions, Genetic; Transcription Factors/metabolism; Transcription, Genetic
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:ABRB		GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000314:		P		Figure 3 shows that “AbrB is a direct repressor of yvcA and yuaB transcription.” Organism: Bacillus subtilis	complete CACAO 13177
BACSU:SP0A		GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000315:		P		In Bacillus subtilis, the deletion of spo0A “resulted in an 11-fold (P <0.01) decrease in transcription.” Figure 2B showed that “a mutant allele of spo0A encoding a version of Spo0A that cannot be phosphorylated resulted in fivefold- decreased yvcA transcription during stationary phase (P 0.01).”	complete CACAO 13136
BACSU:DEGU		GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000315:		P		“Deletion of degU resulted in an eightfold decrease in yuaB transcription which could not be complemented by ectopic expression of a mutant allele of degU that cannot be phosphorylated due to a point mutation in the amino acid that is phosphorylated by DegS (degU-D56N) (Fig. 1C).” Organism: Bacillus subtilis	complete CACAO 13137
BACSU:SINR		GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000315:		P		Figure 4 shows a deletion of sinR in Bacillus subtilis “resulted in a fourfold increase in transcription from the yvcA promoter during exponential growth.”	complete CACAO 13135

References

See Help:References for how to manage references in GONUTS.