PMID:18722549

Citation	Cheng, Y and Hauton, D (2008) Cold acclimation induces physiological cardiac hypertrophy and increases assimilation of triacylglycerol metabolism through lipoprotein lipase. Biochim. Biophys. Acta 1781:618-26
Abstract	The contribution of triacylglycerol to energy provision in the hypertrophied heart, mediated through lipoprotein lipase (LPL) is largely unknown and the contribution of very-low-density lipoprotein (VLDL) receptor to control of LPL presentation at the endothelium is unclear. For isolated perfused rat hearts, cold acclimation (CA) induced volume-overload hypertrophy, with decreased developed pressure (P<0.01), increased end-diastolic volume of the left ventricle (P<0.001) and a loss of contractile reserve in response to dobutamine challenge (P<0.01). Oleate utilisation by perfused hearts was unchanged by CA, however uptake of intralipid emulsion increased 3-fold (P<0.01). CA increased the proportion of lipid deposited in tissue lipids from 10% in euthermic controls to 40% (P<0.01) although the overall contribution of individual lipid classes was unaffected. Cold acclimation significantly increased heparin-releasable LPL (P<0.05) and tissue residual LPL (P<0.01). Western blot analysis indicated preserved expression of proteins coding for SERCA2, muscle-CPT1 and VLDL-receptor following CA, while AMPKalpha2 and phospho-AMPKalpha2 were unaffected. These observations indicate that for physiological hypertrophy AMPK phosphorylation does not mediate the enhanced translocation of LPL to cardiac endothelium.
Links	PubMed PMC2568868 Online version:10.1016/j.bbalip.2008.07.006
Keywords	AMP-Activated Protein Kinases/metabolism; Acclimatization/physiology; Animals; Blood Pressure/drug effects; Blood Pressure/physiology; Body Weight/physiology; Cardiomegaly/metabolism; Carnitine O-Palmitoyltransferase/metabolism; Cholesterol Esters/metabolism; Cold Temperature; Diglycerides/metabolism; Dobutamine/pharmacology; Fat Emulsions, Intravenous/metabolism; Heart/anatomy & histology; Heart/drug effects; Heart/physiology; Lipoprotein Lipase/metabolism; Male; Myocardium/enzymology; Myocardium/metabolism; Oleic Acid/metabolism; Organ Size/drug effects; Oxidation-Reduction; Perfusion; Rats; Rats, Wistar; Receptors, LDL/metabolism; Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism; Triglycerides/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:LIPL	involved_in	GO:0009409: response to cold	ECO:0000270: expression pattern evidence used in manual assertion		P		Seeded From UniProt	complete

References

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