PMID:18650210

Citation	Saito, N, Munemasa, S, Nakamura, Y, Shimoishi, Y, Mori, IC and Murata, Y (2008) Roles of RCN1, regulatory A subunit of protein phosphatase 2A, in methyl jasmonate signaling and signal crosstalk between methyl jasmonate and abscisic acid. Plant Cell Physiol. 49:1396-401
Abstract	Methyl jasmonate (MeJA) as well as abscisic acid (ABA) induces stomatal closure with their signal crosstalk. We investigated the function of a regulatory A subunit of protein phosphatase 2A, RCN1, in MeJA signaling. Both MeJA and ABA failed to induce stomatal closure in Arabidopsis rcn1 knockout mutants unlike in wild-type plants. Neither MeJA nor ABA induced reactive oxygen species (ROS) production and suppressed inward-rectifying potassium channel activities in rcn1 mutants but not in wild-type plants. These results suggest that RCN1 functions upstream of ROS production and downstream of the branch point of MeJA signaling and ABA signaling in Arabidopsis guard cells.
Links	PubMed Online version:10.1093/pcp/pcn106
Keywords	Abscisic Acid/metabolism; Acetic Acids/metabolism; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cyclopentanes/metabolism; Okadaic Acid/pharmacology; Oxylipins/metabolism; Plant Stomata/drug effects; Plant Stomata/metabolism; Potassium Channels, Inwardly Rectifying/metabolism; Protein Phosphatase 2/genetics; Protein Phosphatase 2/metabolism; Reactive Oxygen Species/metabolism; Signal Transduction
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:2AAA		GO:0009738 : abscisic acid mediated signaling pathway	ECO:0000315:		P		Fig.1 Responds to ABA stimulus and drives stomatal movement and ROS production	complete
ARATH:2AAA		GO:0090333 : regulation of stomatal closure	ECO:0000315:		P		Fig.1 ABA-induced stomatal closure was impaired in rcn1 mutant	complete
ARATH:2AAA		GO:0009737 : response to abscisic acid stimulus	ECO:0000315:		P		Fig.1 ABA-induced stomatal closure was impaired in rcn1 mutant	complete
ARATH:2AAA		GO:0009867 : jasmonic acid mediated signaling pathway	ECO:0000315:		P		Fig.2B RCN1 functions upstream of ROS production as a positive transducer of MeJA signaling in guard cells	complete
ARATH:2AAA		GO:0080010 : regulation of oxygen and reactive oxygen species metabolic process	ECO:0000315:		P		Fig.1 Neither MeJA nor ABA promoted ROS production in rcn1 guard cells unlike wild-type guard cells.	complete
ARATH:2AAA		GO:0009753 : response to jasmonic acid stimulus	ECO:0000315:		P		Fig.1	complete

References

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