PMID:18439907

Citation	Hong, S, Lee, S, Cho, SG and Kang, S (2008) UbcH6 interacts with and ubiquitinates the SCA1 gene product ataxin-1. Biochem. Biophys. Res. Commun. 371:256-60
Abstract	UbcH6 is a member of an evolutionally conserved subfamily of E2 ubiquitin-conjugating enzymes. In this study, we report that UbcH6 interacts with and ubiquitinates ataxin-1, the spinocerebellar ataxia type 1 gene product. UbcH6 was identified as an ataxin-1-interacting protein using a yeast two-hybrid screen. UbcH6 co-immunoprecipitates and co-localizes with the ataxin-1 protein in the nucleus. Our binding assays showed that ataxin-1 interacts with UbcH6 through its AXH domain. Interestingly, UbcH6 could ubiquitinate ataxin-1 in the absence of an E3 ligase. The expression level of UbcH6 regulated the rate of ataxin-1 degradation. This study demonstrates that UbcH6 and ataxin-1 are E2-substrate cognate pairs in the ubiquitin-proteasome system.
Links	PubMed Online version:10.1016/j.bbrc.2008.04.066
Keywords	Cell Line; Cell Nucleus/chemistry; Cell Nucleus/metabolism; Humans; Immunoprecipitation; Nerve Tissue Proteins/analysis; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Nuclear Proteins/analysis; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Proteasome Endopeptidase Complex/metabolism; Protein Structure, Tertiary; Two-Hybrid System Techniques; Ubiquitin-Conjugating Enzymes/analysis; Ubiquitin-Conjugating Enzymes/genetics; Ubiquitin-Conjugating Enzymes/metabolism; Ubiquitination
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:UB2E1		GO:0042787: protein ubiquitination involved in ubiquitin-dependent protein catabolic process	ECO:0000315:		P		Figure 4	complete

References

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