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PMID:18023966
| Citation |
Shah, GR, Karunakaran, R and Naresh Kumar, G In vivo restriction endonuclease activity of the Anabaena PCC 7120 XisA protein in Escherichia coli. Res. Microbiol. 158:679-84 |
|---|---|
| Abstract |
Anabaena PCC 7120 genome contains three elements, which get excised out during late stages of heterocyst differentiation by a site-specific recombination process. The XisA protein, which excises the nifD element, shows sequence homology with the integrase family of tyrosine recombinase. The 11 bp target site of XisA CGGAGTAATCC contains a 3 bp inverted repeat. Here, we report restriction endonuclease activity of XisA by specific loss of plasmids containing single or double target sites. The pMX25 plasmid containing two target sites demonstrated endonuclease activity proportional to excision frequency. Different plasmid substrates containing one base pair mutation in the inverted repeat of the target site were monitored for endonuclease activity. Mutation of A4C retained endonuclease activity, while other modifications lost endonuclease activity. The presence of an additional copy of the target site enhanced endonuclease activity. These results suggest that the XisA protein could be an IIE type of restriction endonuclease in addition to being a recombinase. |
| Links |
PubMed Online version:10.1016/j.resmic.2007.08.004 |
| Keywords |
Anabaena/enzymology; Bacterial Proteins/metabolism; DNA Restriction Enzymes/physiology; Escherichia coli/genetics; Integrases/metabolism; Plasmids |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0004519: endonuclease activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0004519: endonuclease activity |
ECO:0000314: |
F |
Table 4 shows specific activity for the 11 base pair target site in artificial substrate plasmid NG7. Endonuclease activity is demonstrated via the loss of substrate plasmid, but not the XisA plasmid, which does not contain a target site. |
complete | ||||
See also
References
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