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NOSS1:XISA

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Species (Taxon ID) Nostoc sp. (strain PCC 7120 / UTEX 2576). (103690)
Gene Name(s) xisA
Protein Name(s) Excisase A

NifD element site-specific recombinase

External Links
UniProt P08862
EMBL U38537
BA000019
PIR A24782
AG1986
RefSeq NP_485485.1
WP_010995614.1
ProteinModelPortal P08862
STRING 103690.alr1442
EnsemblBacteria BAB73399
GeneID 1105037
KEGG ana:alr1442
PATRIC 22772876
eggNOG COG0582
HOGENOM HOG000058475
OMA ININRAL
OrthoDB EOG6NGVNB
Proteomes UP000002483
GO GO:0003677
GO:0015074
GO:0006310
GO:0006281
GO:0043158
Gene3D 1.10.443.10
InterPro IPR011010
IPR013762
IPR002104
Pfam PF00589
SUPFAM SSF56349

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0006310

DNA recombination

PMID:3144039[1]

ECO:0000315

P

Figure 2: DW12-2.5 has a copy of the wild type XisA gene and the inactivated XisA gene, but uses the wild type XisA gene to perform DNA recombination. DW12-2.2 cannot make recombination happen because it only has an inactive XisA gene.

complete
CACAO 8539

GO:0009399

nitrogen fixation

PMID:3144039[1]

ECO:0000315

P

Figure 2: DW12-2.5 has a copy of the wild type XisA gene and the inactivated XisA gene, but uses the wild type XisA gene to perform DNA recombination. DW12-2.2 cannot make recombination happen because it only has an inactive XisA gene.

complete
CACAO 8550

GO:0004519

endonuclease activity

PMID:18023966[2]

ECO:0000314

F

Table 4 shows specific activity for the 11 base pair target site in artificial substrate plasmid NG7. Endonuclease activity is demonstrated via the loss of substrate plasmid, but not the XisA plasmid, which does not contain a target site.

complete
CACAO 8673

involved_in

GO:0009399

nitrogen fixation

PMID:3144039[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006310

DNA recombination

PMID:3144039[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0004519

endonuclease activity

PMID:18023966[2]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0090305

nucleic acid phosphodiester bond hydrolysis

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0004519

P

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR011010
InterPro:IPR013762

F

Seeded From UniProt

complete

involved_in

GO:0006310

DNA recombination

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR013762

P

Seeded From UniProt

complete

involved_in

GO:0015074

DNA integration

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR013762

P

Seeded From UniProt

complete

involved_in

GO:0006281

DNA repair

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0228

P

Seeded From UniProt

complete

involved_in

GO:0043158

heterocyst differentiation

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0364

P

Seeded From UniProt

complete

involved_in

GO:0006310

DNA recombination

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0233

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 Golden, JW & Wiest, DR (1988) Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene. Science 242 1421-3 PubMed GONUTS page
  2. 2.0 2.1 Shah, GR et al. () In vivo restriction endonuclease activity of the Anabaena PCC 7120 XisA protein in Escherichia coli. Res. Microbiol. 158 679-84 PubMed GONUTS page