PMID:17535890

Citation	Wang, YP, Biernat, J, Pickhardt, M, Mandelkow, E and Mandelkow, EM (2007) Stepwise proteolysis liberates tau fragments that nucleate the Alzheimer-like aggregation of full-length tau in a neuronal cell model. Proc. Natl. Acad. Sci. U.S.A. 104:10252-7
Abstract	Tau is a highly soluble protein, yet it aggregates abnormally in Alzheimer's disease. Here, we address the question of proteolytic processing of tau and the nucleation of aggregates by tau fragments. We show in neuronal cell models that fragments of the repeat domain of tau containing mutations of FTDP17 (frontotemporal dementia with parkinsonism linked to chromosome 17), produced by endogenous proteases, can induce the aggregation of full-length tau. Fragments are generated by successive cleavages, first N-terminally between K257 and S258, then C-terminally around residues 353-364; conversely, when the N-terminal cleavage is inhibited, no fragmentation and aggregation takes place. The C-terminal truncation and the coaggregation of fragments with full-length tau depends on the propensity for beta-structure. The aggregation is modulated by phosphorylation but does not depend on it. Aggregation but not fragmentation as such is toxic to cells; conversely, toxicity can be prevented by inhibiting either aggregation or proteolysis. The results reveal a novel pathway of abnormal tau aggregation in neuronal cells.
Links	PubMed PMC1891218 Online version:10.1073/pnas.0703676104
Keywords	Alzheimer Disease/metabolism; Amino Acid Motifs; Amino Acid Sequence; Amino Acid Substitution; Animals; Binding Sites; Cell Line, Tumor; Fluorescent Antibody Technique, Indirect; Humans; Hydrolysis; Mice; Microscopy, Fluorescence; Models, Biological; Models, Chemical; Neuroblastoma/pathology; Neurons/metabolism; Peptide Fragments/metabolism; Peptide Hydrolases/chemistry; Peptide Hydrolases/metabolism; Phosphorylation; Protein Binding; Protein Conformation; Protein Isoforms; Protein Processing, Post-Translational; Protein Structure, Secondary; Protein Structure, Tertiary; Solubility; Thrombin/chemistry; tau Proteins/chemistry; tau Proteins/genetics; tau Proteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:TAU	enables	GO:0042802: identical protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:P10636-8	F		Seeded From UniProt	complete

References

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