PMID:17499043

Citation	Johnson, SS, Zhang, C, Fromm, J, Willis, IM and Johnson, DL (2007) Mammalian Maf1 is a negative regulator of transcription by all three nuclear RNA polymerases. Mol. Cell 26:367-79
Abstract	Most eukaryotic transcriptional regulators act in an RNA polymerase (Pol)-selective manner. Here we show that the human Maf1 protein negatively regulates transcription by all three nuclear Pols. Changes in Maf1 expression affect Pol I- and Pol III-dependent transcription in human glioblastoma lines. These effects are mediated, in part, through the ability of Maf1 to repress transcription of the TATA binding protein, TBP. Maf1 targets an Elk-1-binding site in the TBP promoter, and its occupancy of this region is reciprocal with that of Elk-1. Similarly, Maf1 occupancy of Pol III genes is inversely correlated with that of the initiation factor TFIIIB and Pol III. The phenotypic consequences of reducing Maf1 expression include changes in cell morphology and the accumulation of actin stress fibers, whereas Maf1 overexpression suppresses anchorage-independent growth. Together with the ability of Maf1 to reduce biosynthetic capacity, these findings support the idea that Maf1 regulates the transformation state of cells.
Links	PubMed Online version:10.1016/j.molcel.2007.03.021
Keywords	Binding Sites; Cell Growth Processes; Cell Nucleus/genetics; Cell Nucleus/metabolism; Cell Transformation, Neoplastic/genetics; Cell Transformation, Neoplastic/metabolism; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Gene Expression Regulation; Humans; Promoter Regions, Genetic/genetics; Protein Binding; RNA Interference; RNA Polymerase I/genetics; RNA Polymerase I/metabolism; RNA Polymerase II/genetics; RNA Polymerase II/metabolism; RNA Polymerase III/genetics; RNA Polymerase III/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; TATA-Binding Protein Associated Factors/genetics; TATA-Binding Protein Associated Factors/metabolism; TATA-Box Binding Protein/genetics; TATA-Box Binding Protein/metabolism; Transcription Factor TFIIIB/genetics; Transcription Factor TFIIIB/metabolism; Transcription, Genetic/physiology; Tumor Cells, Cultured
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:MAF1		GO:0016479: negative regulation of transcription from RNA polymerase I promoter	ECO:0000314:		P		The author analyzed the effect of increasing and decreasing the transcription of MAF1 on polymerase 1 dependent transcription. It was found that RNA polymerase 1 dependent transcription was decreased in the MAF1 upregulated cells (Figure 1)	complete CACAO 12192
HUMAN:MAF1		GO:0016480: negative regulation of transcription from RNA polymerase III promoter	ECO:0000314:		P		The author analyzed the effect of increasing and decreasing the transcription of MAF1 on polymerase 3 dependent transcription. It was found that RNA polymerase 3 dependent transcription was decreased in the MAF1 upregulated cells (Figure 1)	complete CACAO 12193

References

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