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PMID:1729245
| Citation |
Chakraborty, T, Leimeister-Wächter, M, Domann, E, Hartl, M, Goebel, W, Nichterlein, T and Notermans, S (1992) Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA gene. J. Bacteriol. 174:568-74 |
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| Abstract |
The prfA gene of Listeria monocytogenes encodes a protein that activates transcription of the listeriolysin gene (lisA). In order to explore the role of the prfA gene product in the pathogenesis of listerial infection, we constructed a site-directed insertion mutation in prfA by the chromosomal integration of a novel suicide vector containing a portion of the prfA coding region. This mutation not only transcriptionally silenced the listeriolysin (lisA) gene but also abrogated production of specific RNA transcripts corresponding to the phosphatidylinositol-specific phospholipase C (pic) and metalloprotease (mpl) genes, two further virulence gene products expressed only by pathogenic Listeria strains. The strain was also found to be avirulent when tested in a mouse model of listerial infection. The concomitant loss of multiple characteristics such as production of LisA, Pic, Mpl, and loss of virulence in a mouse infection model is the result of a mutation in a single gene and demonstrates that the prfA gene product is a positive regulator of multiple virulence determinants in L. monocytogenes. |
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| Keywords |
Animals; Biological Assay; Genes, Bacterial; Listeria monocytogenes/genetics; Listeria monocytogenes/growth & development; Listeria monocytogenes/pathogenicity; Listeriosis/genetics; Listeriosis/microbiology; Mice; Mutagenesis, Insertional; Plasmids; Spleen; Virulence |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0009405: pathogenesis |
ECO:0000315: |
P |
Figure 6. shows that prfA mutant strains EGD prfAI and SLCC 53 prfA were less virulent and exhibited reduced growth in host tissue. |
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References
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