PMID:17254795

Citation	Ferrari, F, Mercaldo, V, Piccoli, G, Sala, C, Cannata, S, Achsel, T and Bagni, C (2007) The fragile X mental retardation protein-RNP granules show an mGluR-dependent localization in the post-synaptic spines. Mol. Cell. Neurosci. 34:343-54
Abstract	The localization of RNA/mRNA in dendrites plays a role in both local and temporal regulation of protein synthesis, which is required for certain forms of synaptic plasticity. A key molecule in these processes is the fragile X mental retardation protein (FMRP). Using in situ hybridization coupled to immunofluorescence confocal microscopy, we find that the FMRP-RNP particle contains alphaCaMKII and BC1 RNAs as well as Staufen and CPEB proteins. Furthermore, following mGluR activation, the FMRP-mRNP complex moves into spines as shown by co-localization with the PSD-95 and Shank proteins. This study shows, for the first time, that the translationally inactive FMRP-mRNP complex relocates into neuronal spines after stimulation and that de novo protein synthesis mainly contributes to the pool of FMRP at synapses.
Links	PubMed Online version:10.1016/j.mcn.2006.11.015
Keywords	Animals; Brain/cytology; Cells, Cultured; Cercopithecus aethiops; Cricetinae; Dendritic Spines/drug effects; Dendritic Spines/metabolism; Disks Large Homolog 4 Protein; Embryo, Mammalian; Fluorescent Antibody Technique/methods; Fragile X Mental Retardation Protein/metabolism; Gene Expression Regulation, Developmental/drug effects; Gene Expression Regulation, Developmental/physiology; Guanylate Kinases; In Situ Hybridization; Intracellular Signaling Peptides and Proteins/metabolism; Membrane Proteins/metabolism; Methoxyhydroxyphenylglycol/analogs & derivatives; Methoxyhydroxyphenylglycol/pharmacology; Mice; Microtubule-Associated Proteins/metabolism; Nerve Tissue Proteins/metabolism; Neurons/cytology; RNA-Binding Proteins/metabolism; Receptors, Metabotropic Glutamate/antagonists & inhibitors; Receptors, Metabotropic Glutamate/physiology; Time Factors; Transfection; tau Proteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:FMR1	part_of	GO:0098794: postsynapse	ECO:0000314: direct assay evidence used in manual assertion		C	part_of:(CL:0002608) part_of:(GO:0043197)	Seeded From UniProt	complete

References

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