PMID:17220230

Citation	Masayama, A, Kuwana, R, Takamatsu, H, Hemmi, H, Yoshimura, T, Watabe, K and Moriyama, R (2007) A novel lipolytic enzyme, YcsK (LipC), located in the spore coat of Bacillus subtilis, is involved in spore germination. J. Bacteriol. 189:2369-75
Abstract	The predicted amino acid sequence of Bacillus subtilis ycsK exhibits similarity to the GDSL family of lipolytic enzymes. Northern blot analysis showed that ycsK mRNA was first detected from 4 h after the onset of sporulation and that transcription of ycsK was dependent on SigK and GerE. The fluorescence of the YcsK-green fluorescent protein fusion protein produced in sporulating cells was detectable in the mother cell but not in the forespore compartment under fluorescence microscopy, and the fusion protein was localized around the developing spores dependent on CotE, SafA, and SpoVID. Inactivation of the ycsK gene by insertion of an erythromycin resistance gene did not affect vegetative growth or spore resistance to heat, lysozyme, or chloroform. The germination of ycsK spores in a mixture of L-asparagine, D-glucose, D-fructose, and potassium chloride and LB medium was also the same as that of wild-type spores, but the mutant spores were defective in L-alanine-stimulated germination. In addition, zymogram analysis demonstrated that the YcsK protein heterologously expressed in Escherichia coli showed lipolytic activity. We therefore propose that ycsK should be renamed lipC. This is the first study of a bacterial spore germination-related lipase.
Links	PubMed PMC1899377 Online version:10.1128/JB.01527-06
Keywords	Amino Acid Sequence; Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Bacillus subtilis/physiology; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Gene Expression Regulation, Bacterial; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Lipase/chemistry; Lipase/genetics; Lipase/metabolism; Microscopy, Fluorescence; Molecular Sequence Data; RNA, Messenger/genetics; RNA, Messenger/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Sequence Analysis, DNA; Spores, Bacterial/enzymology; Spores, Bacterial/genetics; Spores, Bacterial/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:LIPC		GO:0016042: lipid catabolic process	ECO:0000314:		P		Figure 6 shows an SDS & zymography assay performed using an empty vector, a vector with YtsK, and bovine serum albumin. Lane 2 is YtsK's SDS and shows the protein's molecular weight. Lane 5 is YtsK's zymography assay and it shows lysis activity at the same kDa as in the SDS indicating YtsK has lypolytic activity. The control's zymogen assay showed no activity.	complete CACAO 9970
BACSU:LIPC	involved_in	GO:0016042: lipid catabolic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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