GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:17110038
| Citation |
Deshpande, LS, Sombati, S, Blair, RE, Carter, DS, Martin, BR and DeLorenzo, RJ (2007) Cannabinoid CB1 receptor antagonists cause status epilepticus-like activity in the hippocampal neuronal culture model of acquired epilepsy. Neurosci. Lett. 411:11-6 |
|---|---|
| Abstract |
Status epilepticus (SE) is a major medical emergency associated with a significant morbidity and mortality. Little is known about the mechanisms that terminate seizure activity and prevent the development of status epilepticus. Cannabinoids possess anticonvulsant properties and the endocannabinoid system has been implicated in regulating seizure duration and frequency. Endocannabinoids regulate synaptic transmission and dampen seizure activity via activation of the presynaptic cannabinoid receptor 1 (CB1). This study was initiated to evaluate the role of CB1 receptor-dependent endocannabinoid synaptic transmission towards preventing the development of status epilepticus-like activity in the well-characterized hippocampal neuronal culture model of acquired epilepsy using patch clamp electrophysiology. Application of the CB1 receptor antagonists SR141716A (1 microM) or AM251 (1 microM) to "epileptic" neurons caused the development of continuous epileptiform activity, resembling electrographic status epilepticus. The induction of status epilepticus-like activity by CB1 receptor antagonists was reversible and could be overcome by maximal concentrations of CB1 agonists. Similar treatment of control neurons with CB1 receptor antagonists did not produce status epilepticus or hyperexcitability. These findings suggest that CB1 receptor-dependent endocannabinoid endogenous tone plays an important role in modulating seizure frequency and duration and preventing the development of status epilepticus-like activity in populations of epileptic neurons. The regulation of seizure activity and prevention of status epilepticus by the endocannabinoid system offers an important insight into understanding the basic mechanisms that control the development of continuous epileptiform discharges. |
| Links |
PubMed PMC1808496 Online version:10.1016/j.neulet.2006.09.046 |
| Keywords |
Action Potentials/drug effects; Action Potentials/physiology; Action Potentials/radiation effects; Animals; Animals, Newborn; Benzoxazines; Cells, Cultured; Disease Models, Animal; Drug Interactions; Epilepsy/chemically induced; Epilepsy/pathology; Hippocampus/pathology; Morpholines/pharmacology; Naphthalenes/pharmacology; Neurons/drug effects; Neurons/physiology; Patch-Clamp Techniques/methods; Piperidines/pharmacology; Pyrazoles/pharmacology; Rats; Rats, Sprague-Dawley; Receptor, Cannabinoid, CB1/antagonists & inhibitors; Synaptic Transmission/drug effects; Synaptic Transmission/physiology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0007186: G protein-coupled receptor signaling pathway |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004949: cannabinoid receptor activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008144: drug binding |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
Notes
See also
References
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