PMID:1703156

Citation	Hollingsworth, HC and Nossal, NG (1991) Bacteriophage T4 encodes an RNase H which removes RNA primers made by the T4 DNA replication system in vitro. J. Biol. Chem. 266:1888-97
Abstract	RNase H activity increases markedly after bacteriophage T4 infection of Escherichia coli MIC2003, an RNase H-deficient host. We have extensively purified the RNase H from these T4-infected cells and have shown that the RNase H activity copurifies with a 5' to 3' DNA exonuclease activity. The N-terminal sequence of a 35-kDa protein copurifying with the RNase H activity matches the terminus of the predicted product of an open reading frame (designated ORF A or 33.2) upstream of T4 gene 33, identified previously by Hahn and co-workers (Hahn, S., Kruse, U., and Rüger, W. (1986) Nucleic Acids Res. 14, 9311-9327). Plasmids containing ORF A under the control of the T7 promoter express RNase H and 5' to 3' DNA exonuclease activities as well as a protein that comigrates on sodium dodecyl sulfate-polyacrylamide gels with the 35-kDa protein present in the RNase H purified from T4-infected cells. T4 RNase H removes the pentamer RNA primers from DNA chains initiated by the T4 primase-helicase (gene products 61 and 41). Addition of T4 RNase H and T4 DNA ligase leads to extensive joining of discontinuous lagging strand fragments in the T4 DNA replication system in vitro.
Links	PubMed
Keywords	Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA Primase; DNA Replication; DNA, Viral/biosynthesis; Endoribonucleases/genetics; Endoribonucleases/metabolism; Exodeoxyribonucleases/metabolism; Genes, Viral; In Vitro Techniques; Molecular Sequence Data; Molecular Weight; Oligonucleotides/metabolism; RNA/metabolism; RNA Nucleotidyltransferases/metabolism; Ribonuclease H; Substrate Specificity; T-Phages/enzymology; T-Phages/genetics; Viral Structural Proteins/genetics; Virus Replication
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BPT4:RNH		GO:0004534: 5'-3' exoribonuclease activity	ECO:0000314:		F		FIG. 4. Oligonucleotide products of the 5’ to 3’ DNA exonuclease activity of T4 RNase H	complete CACAO 11942
BPT4:RNH		GO:0004533: exoribonuclease H activity	ECO:0000314:		F		FIG. 1	complete
BPT4:RNH		GO:0043137: DNA replication, removal of RNA primer	ECO:0000314:		P		Fig 5 -- Removal of RNA primers radiolabeled with phosphorus was quantified by gel electrophoresis.	complete CACAO 11989

References

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