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Masters, RA, Crean, BD, Yan, W, Moss, AG, Ryan, PL, Wiley, AA, Bagnell, CA and Bartol, FF (2007) Neonatal porcine endometrial development and epithelial proliferation affected by age and exposure to estrogen and relaxin. Domest. Anim. Endocrinol. 33:335-46


In the pig, temporospatially regulated proliferation of uterine luminal (LE) and glandular (GE) epithelium between birth (postnatal day=PND 0) and PND 15 is essential for success of endometrial development. Exposure of gilts to estrogen (E) or relaxin (RLX) during this period disrupts uterine development, and neonatal E exposure can compromise adult uterine function. Neonatal uterotrophic effects of E and RLX, administered for 2 days beginning on PND 12, can be inhibited with the antiestrogen ICI 182,780 (ICI) indicating crosstalk between RLX and E signaling systems. Here, objectives were to determine effects of: (study 1) neonatal age and (study 2) exposure to E, RLX, and ICI on porcine neonatal uterine histoarchitecture and patterns of epithelial cell proliferation as reflected by proliferating cell nuclear antigen labeling index. In study 1, uteri were obtained on PND 0, 3, 6, 9, 12 and 15. Glandular epithelium, absent at birth, was observed by PND 3. Overall, epithelial labeling index increased from birth to PND 3, declined from PND 6-9 in LE and GE, and increased to PND 15 in GE. In study 2, uteri were collected on PND 14 after administration of vehicle, E, or RLX for 2 days, or following pretreatment with ICI. Alone, E was uterotrophic and adenogenic and increased labeling index in both LE and GE. Both RLX and ICI increased proliferation in GE. Effects of E and RLX were attenuated by ICI, providing further support for crosstalk between these signaling systems in the developing neonatal porcine endometrium.


PubMed Online version:10.1016/j.domaniend.2006.07.002


Age Factors; Animals; Animals, Newborn; Cell Growth Processes/drug effects; Endometrium/cytology; Endometrium/drug effects; Endometrium/growth & development; Epithelial Cells/cytology; Epithelial Cells/drug effects; Estradiol/analogs & derivatives; Estradiol/pharmacology; Estrogen Antagonists/pharmacology; Female; Image Processing, Computer-Assisted; Immunohistochemistry/veterinary; Linear Models; Proliferating Cell Nuclear Antigen/metabolism; Random Allocation; Relaxin/pharmacology; Swine/physiology



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status



GO:0050679: positive regulation of epithelial cell proliferation

ECO:0000314: direct assay evidence used in manual assertion


Seeded From UniProt



GO:0050679: positive regulation of epithelial cell proliferation



In figure 6, the authors demonstrate that porcine relaxin increases the proportion of cells that are positive for proliferating cell nuclear antigen, indicating cell proliferation.

CACAO 2051


GO:0033148: positive regulation of intracellular estrogen receptor signaling pathway



Fig. 6


See also


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