PMID:16950411

Citation	Cheng, BC, Chang, CP, Lin, MT and Lee, CC (2007) Inhibition of neuronal nitric oxide synthase causes attenuation of cerebrovascular dysfunction in experimental heatstroke. Neuropharmacology 52:297-305
Abstract	The present study was performed to assess the prophylactic effect of 7-nitroindazole (7-NI), an inhibitor of neuronal nitric oxide synthase (nNOS), in an animal model of heatstroke. Anesthetized rats, immediately before the start of heat stress, were divided into two major groups and given the following: vehicle solution (1 mL per kg body weight) or 7-NI (5-20mg/mL per kg body weight) intraperitoneally. They were exposed to ambient temperature of 43 degrees C to induce heatstroke. Another group of rats were exposed to room temperature (24 degrees C) and used as normothermic controls. Their physiologic and biochemical parameters were continuously monitored. When the vehicle-pretreated rats underwent heat stress, their survival time values were found to be 21-25 min. Pretreatment with intraperitoneal doses of 7-NI significantly improved survival during heatstroke (55-164 min). As compared to those of normothermic controls, all vehicle-pretreated heatstroke animals displayed higher levels of core temperature, intracranial pressure, nitric oxide metabolite (NO(2)(-)), glutamate, glycerol, lactate/pyruvate ratio, neuronal damage score and nNOS expression in the hypothalamus, and tumor necrosis factor-alpha (TNF-alpha) in the serum. In contrast, all vehicle-pretreated heatstroke animals had lower levels of mean arterial pressure, cerebral perfusion pressure, cerebral blood flow, and brain PO(2). Administration of 7-NI before the start of heat exposure significantly reduced the hyperthermia, intracranial hypertension, nNOS-dependent NO(2)(-), glutamate, glycerol, lactate/pyruvate ratio, and neuronal damage score in the hypothalamus, as well as overproduction of TNF-alpha in the serum that occurred during heatstroke. The data show that reduction of nNOS-dependent NO(2)(-) with 7-NI causes attenuation of cerebrovascular dysfunction, hyperthermia, and TNF-alpha overproduction during heatstroke in the rat.
Links	PubMed Online version:10.1016/j.neuropharm.2006.07.033
Keywords	Animals; Blood Pressure/drug effects; Body Temperature/drug effects; Brain Chemistry/drug effects; Cerebrovascular Disorders/enzymology; Cerebrovascular Disorders/etiology; Cerebrovascular Disorders/pathology; Cerebrovascular Disorders/prevention & control; Disease Models, Animal; Enzyme Inhibitors/administration & dosage; Heat Stroke/complications; Hypothalamus/enzymology; Immunohistochemistry/methods; Indazoles/administration & dosage; Intracranial Pressure/drug effects; Neural Inhibition/drug effects; Neural Inhibition/physiology; Nitric Oxide Synthase Type I/metabolism; Rats; Rats, Sprague-Dawley; Time Factors; Tumor Necrosis Factor-alpha/blood
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:NOS1	involved_in	GO:0009408: response to heat	ECO:0000270: expression pattern evidence used in manual assertion		P		Seeded From UniProt	complete

References

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