PMID:16926527

Citation	Miah, AG, Tareq, KM, Hamano, K, Kohsaka, T and Tsujii, H (2006) Effect of relaxin on acrosome reaction and utilization of glucose in boar spermatozoa. J. Reprod. Dev. 52:773-9
Abstract	Relaxin is a peptide hormone found in seminal plasma that has a physiological influence on sperm motility in some species. There are no reports on the effect of relaxin on acrosome reaction and utilization of glucose in boar spermatozoa. In this study, to investigate the effects of relaxin on sperm motility, acrosome reaction, and incorporation and oxidation of labeled glucose, boar spermatozoa were washed and preincubated for swim-up and then incubated (0-6 h) with 0, 20, or 40 ng/ml relaxin in mTALP medium. The results indicated that the addition of relaxin stimulated sperm motility significantly (P<0.05) during 1-4 h of incubation. The percentage of acrosome-reacted live spermatozoa was higher (P<0.05) when the spermatozoa were treated with 20 or 40 ng/ml relaxin. The rate of incorporation, and oxidation of glucose were also greater (P<0.05) in the spermatozoa incubated with relaxin compared to the control spermatozoa. The rate of incorporation and oxidation of (14)C-glucose were increased in correlation with acrosome reaction up to 4 h of incubation and then decreased in line with the increasing incubation period. In conclusion, the present study demonstrates that relaxin accelerates not only motility but also the acrosome reaction and utilization of glucose in boar spermatozoa.
Links	PubMed
Keywords	Acrosome Reaction/drug effects; Acrosome Reaction/physiology; Animals; Female; Glucose/metabolism; Male; Relaxin/metabolism; Relaxin/pharmacology; Relaxin/physiology; Sperm Motility/drug effects; Sperm Motility/physiology; Spermatozoa/drug effects; Spermatozoa/metabolism; Spermatozoa/physiology; Swine/metabolism; Swine/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PIG:RELX		GO:2000344: positive regulation of acrosome reaction	ECO:0000314:		P		In figure 1, the authors demonstrate that the addition of porcine relaxin increased the proportion of live boar spermatozoa that had undergone the acrosome reaction.	complete CACAO 2004
PIG:RELX	involved_in	GO:0046326: positive regulation of glucose import	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
PIG:RELX		GO:0030317: sperm motility	ECO:0000314:		P		In figure 1, the authors demonstrate that the addition of porcine relaxin increases the motility of boar spermatozoa.	complete CACAO 1958
PIG:RELX		GO:0046326 : positive regulation of glucose import	ECO:0000314:		P		In figure 2, the authors demonstrate that the addition of porcine relaxin increases the glucose import in boar spermatozoa.	complete CACAO 1959
PIG:RELX	involved_in	GO:0030317: flagellated sperm motility	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
PIG:RELX	involved_in	GO:2000344: positive regulation of acrosome reaction	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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