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PMID:16856941
| Citation |
Kentner, D, Thiem, S, Hildenbeutel, M and Sourjik, V (2006) Determinants of chemoreceptor cluster formation in Escherichia coli. Mol. Microbiol. 61:407-17 |
|---|---|
| Abstract |
Chemotactic stimuli in bacteria are sensed by large sensory complexes, or receptor clusters, that consist of tens of thousands of proteins. Receptor clusters appear to play a key role in signal processing, but their structure remains poorly understood. Here we used fluorescent protein fusions to study in vivo formation of the cluster core, which consists of receptors, a kinase CheA and an assisting protein CheW. We show that receptors aggregate through their cytoplasmic domains even in the absence of other chemotaxis proteins. Clustering is further enhanced by the binding of CheW. Surprisingly, we observed that some fragments of CheA bind receptor clusters well in the absence of CheW, although the latter does assist the binding of full-length CheA. The resulting mode of receptor cluster formation is consistent with an experimentally observed flexible stoichiometry of chemosensory complexes and with assumptions of recently proposed computer models of signal processing in chemotaxis. |
| Links |
PubMed Online version:10.1111/j.1365-2958.2006.05250.x |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Chemoreceptor Cells/metabolism; Cytoplasm/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Methyltransferases/genetics; Multiprotein Complexes; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Signal Transduction |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0051649: establishment of localization in cell |
ECO:0000315: |
P |
Figure 1 and Table 1. CheW expression in CheW/CheA knockout cells restored the compact clusters found in CheW+CheA+ cells. CheW/CheA knockout cells had a cap or multiple small clusters instead of a single compact cluster. |
complete | ||||
|
involved_in |
GO:0051649: establishment of localization in cell |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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