PMID:16672615

Citation	Rodina, A and Godson, GN (2006) Role of conserved amino acids in the catalytic activity of Escherichia coli primase. J. Bacteriol. 188:3614-21
Abstract	The role of conserved amino acid residues in the polymerase domain of Escherichia coli primase has been studied by mutagenesis. We demonstrate that each of the conserved amino acids Arg146, Arg221, Tyr230, Gly266, and Asp311 is involved in the process of catalysis. Residues Glu265 and Asp309 are also critical because a substitution of each amino acid irreversibly destroys the catalytic activity. Two K229A and M268A mutant primase proteins synthesize only 2-nucleotide products in de novo synthesis reactions under standard conditions. Y267A mutant primase protein synthesizes both full-size and 2-nucleotide RNA, but with no intermediate-size products. From these data we discuss the significant step of the 2-nucleotide primer RNA synthesis by E. coli primase and the role of amino acids Lys229, Tyr267, and Met268 in primase complex stability.
Links	PubMed PMC1482863 Online version:10.1128/JB.188.10.3614-3621.2006
Keywords	Amino Acid Sequence; Base Sequence; Conserved Sequence; DNA Primase/chemistry; DNA Primase/genetics; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides/chemistry; RNA, Bacterial/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:DNAG		GO:0006269: DNA replication, synthesis of RNA primer	ECO:0000315:		P		Fig. 2	complete CACAO 8286
ECOLI:DNAG	involved_in	GO:0006269: DNA replication, synthesis of RNA primer	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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