PMID:1663890

Citation	Kajie, S, Ideta, R, Yamato, I and Anraku, Y (1991) Molecular cloning and DNA sequence of dniR, a gene affecting anaerobic expression of the Escherichia coli hexaheme nitrite reductase. FEMS Microbiol. Lett. 67:205-11
Abstract	A gene responsible for increased synthesis of hexaheme nitrite reductase (cytochrome c552) of Escherichia coli K-12 was cloned into pBR322 by the direct immunological screening method using antiserum against the purified enzyme. The cloned gene was mapped at 5 min on the chromosomal linkage map as the dni gene (related to increased synthesis of the dissimilatory nitrite reductase) by conjugation and transduction. The dni gene was subcloned into pUC118 and was shown to be on a 2.6-kilobase-pair PstI-BamHI fragment by immunoblotting analysis of the expressed enzyme. The nucleotide sequence of this fragment was determined. A plausible open-reading frame corresponding to 222 amino acids was detected. Analysis of a dni deletion mutant by immunoblotting demonstrated that this mutant expressed a greatly reduced amount of the nitrite reductase. Thus, the dni gene is suggested to have a positive regulatory action on induced synthesis of the nitrite reductase, and was designated as dniR.
Links	PubMed
Keywords	Amino Acid Sequence; Anaerobiosis; Base Sequence; Chromosome Deletion; Cloning, Molecular; Cytochrome c Group/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Genotype; Molecular Sequence Data; Restriction Mapping
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:MLTD	involved_in	GO:0051353: positive regulation of oxidoreductase activity	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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