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PMID:16571833
| Citation |
Schornberg, K, Matsuyama, S, Kabsch, K, Delos, S, Bouton, A and White, J (2006) Role of endosomal cathepsins in entry mediated by the Ebola virus glycoprotein. J. Virol. 80:4174-8 |
|---|---|
| Abstract |
Using chemical inhibitors and small interfering RNA (siRNA), we have confirmed roles for cathepsin B (CatB) and cathepsin L (CatL) in Ebola virus glycoprotein (GP)-mediated infection. Treatment of Ebola virus GP pseudovirions with CatB and CatL converts GP1 from a 130-kDa to a 19-kDa species. Virus with 19-kDa GP1 displays significantly enhanced infection and is largely resistant to the effects of the CatB inhibitor and siRNA, but it still requires a low-pH-dependent endosomal/lysosomal function. These and other results support a model in which CatB and CatL prime GP by generating a 19-kDa intermediate that can be acted upon by an as yet unidentified endosomal/lysosomal enzyme to trigger fusion. |
| Links |
PubMed PMC1440424 Online version:10.1128/JVI.80.8.4174-4178.2006 |
| Keywords |
Animals; Cathepsin B/physiology; Cathepsin L; Cathepsins/physiology; Cercopithecus aethiops; Cricetinae; Cysteine Endopeptidases/physiology; Ebolavirus/physiology; Endosomes/enzymology; RNA, Small Interfering/pharmacology; Vero Cells; Vesicular stomatitis Indiana virus/genetics; Viral Fusion Proteins/physiology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0004197: cysteine-type endopeptidase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0046718: viral entry into host cell |
ECO:0000314: |
P |
Fig. 1A, 1C and Fig. 3(A,B,C) |
complete | ||||
| GO:0004197: cysteine-type endopeptidase activity |
ECO:0000314: |
F |
Fig. 2 |
complete | ||||
|
involved_in |
GO:0046718: viral entry into host cell |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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