PMID:16364653

Citation	Annies, M, Bittcher, G, Ramseger, R, Löschinger, J, Wöll, S, Porten, E, Abraham, C, Rüegg, MA and Kröger, S (2006) Clustering transmembrane-agrin induces filopodia-like processes on axons and dendrites. Mol. Cell. Neurosci. 31:515-24
Abstract	The transmembrane form of agrin (TM-agrin) is primarily expressed in the CNS, particularly on neurites. To analyze its function, we clustered TM-agrin on neurons using anti-agrin antibodies. On axons from the chick CNS and PNS as well as on axons and dendrites from mouse hippocampal neurons anti-agrin antibodies induced the dose- and time-dependent formation of numerous filopodia-like processes. The processes appeared within minutes after antibody addition and contained a complex cytoskeleton. Formation of processes required calcium, could be inhibited by cytochalasine D, but was not influenced by staurosporine, heparin or pervanadate. Time-lapse video microscopy revealed that the processes were dynamic and extended laterally along the entire length of the neuron. The lateral processes had growth cones at their tips that initially adhered to the substrate, but subsequently collapsed and were retracted. These data provide the first evidence for a specific role of TM-agrin in shaping the cytoskeleton of neurites in the developing nervous system.
Links	PubMed Online version:10.1016/j.mcn.2005.11.005
Keywords	Agrin/antagonists & inhibitors; Agrin/metabolism; Animals; Animals, Newborn; Antibodies/pharmacology; Cell Differentiation/physiology; Cell Membrane/drug effects; Cell Membrane/metabolism; Cell Membrane/ultrastructure; Cells, Cultured; Chick Embryo; Cytochalasin B/pharmacology; Cytoskeleton/drug effects; Cytoskeleton/metabolism; Cytoskeleton/ultrastructure; Dendrites/drug effects; Dendrites/metabolism; Dendrites/ultrastructure; Dose-Response Relationship, Drug; Growth Cones/drug effects; Growth Cones/metabolism; Growth Cones/ultrastructure; Mice; Microscopy, Video; Nervous System/cytology; Nervous System/embryology; Nervous System/metabolism; Neurites/drug effects; Neurites/metabolism; Neurites/ultrastructure; Pseudopodia/drug effects; Pseudopodia/metabolism; Pseudopodia/ultrastructure; Time Factors
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CHICK:AGRIN	involved_in	GO:0046847: filopodium assembly	ECO:0000314: direct assay evidence used in manual assertion		P	occurs_in:(CL:0011103)	Seeded From UniProt	complete
CHICK:AGRIN	involved_in	GO:0007399: nervous system development	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
CHICK:AGRIN	part_of	GO:0030424: axon	ECO:0000314: direct assay evidence used in manual assertion		C	part_of:(GO:0009986) part_of:(CL:0000740)	Seeded From UniProt	complete

References

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