PMID:16352847

Citation	González Barrios, AF, Zuo, R, Hashimoto, Y, Yang, L, Bentley, WE and Wood, TK (2006) Autoinducer 2 controls biofilm formation in Escherichia coli through a novel motility quorum-sensing regulator (MqsR, B3022). J. Bacteriol. 188:305-16
Abstract	The cross-species bacterial communication signal autoinducer 2 (AI-2), produced by the purified enzymes Pfs (nucleosidase) and LuxS (terminal synthase) from S-adenosylhomocysteine, directly increased Escherichia coli biofilm mass 30-fold. Continuous-flow cells coupled with confocal microscopy corroborated these results by showing the addition of AI-2 significantly increased both biofilm mass and thickness and reduced the interstitial space between microcolonies. As expected, the addition of AI-2 to cells which lack the ability to transport AI-2 (lsr null mutant) failed to stimulate biofilm formation. Since the addition of AI-2 increased cell motility through enhanced transcription of five motility genes, we propose that AI-2 stimulates biofilm formation and alters its architecture by stimulating flagellar motion and motility. It was also found that the uncharacterized protein B3022 regulates this AI-2-mediated motility and biofilm phenotype through the two-component motility regulatory system QseBC. Deletion of b3022 abolished motility, which was restored by expressing b3022 in trans. Deletion of b3022 also decreased biofilm formation significantly, relative to the wild-type strain in three media (46 to 74%) in 96-well plates, as well as decreased biomass (8-fold) and substratum coverage (19-fold) in continuous-flow cells with minimal medium (growth rate not altered and biofilm restored by expressing b3022 in trans). Deleting b3022 changed the wild-type biofilm architecture from a thick (54-mum) complex structure to one that contained only a few microcolonies. B3022 positively regulates expression of qseBC, flhD, fliA, and motA, since deleting b3022 decreased their transcription by 61-, 25-, 2.4-, and 18-fold, respectively. Transcriptome analysis also revealed that B3022 induces crl (26-fold) and flhCD (8- to 27-fold). Adding AI-2 (6.4 muM) increased biofilm formation of wild-type K-12 MG1655 but not that of the isogenic b3022, qseBC, fliA, and motA mutants. Adding AI-2 also increased motA transcription for the wild-type strain but did not stimulate motA transcription for the b3022 and qseB mutants. Together, these results indicate AI-2 induces biofilm formation in E. coli through B3022, which then regulates QseBC and motility; hence, b3022 has been renamed the motility quorum-sensing regulator gene (the mqsR gene).
Links	PubMed PMC1317603 Online version:10.1128/JB.188.1.305-316.2006
Keywords	Biofilms/growth & development; Culture Media; Escherichia coli/growth & development; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Homoserine/analogs & derivatives; Homoserine/metabolism; Lactones/metabolism; Movement; Signal Transduction
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:MQSR	involved_in	GO:2000145: regulation of cell motility	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:MQSR		GO:2000145: regulation of cell motility	ECO:0000315:		P		Figure 5 shows that a mutation in the mqsR gene leads to almost no qseB transcription (qseB is a quorum sensing motility regulating 2 component regulon protein), as well as motA, another motility related gene.	complete CACAO 5599
ECOLI:MQSR		GO:0045892: negative regulation of transcription, DNA-dependent	ECO:0000315:		P		Figure 5	complete CACAO 5709
ECOLI:MQSR		GO:2000145: quorum sensing	ECO:0000315:		P		Figure 5 shows that a mutation in the mqsR gene leads to almost no qseB transcription (qseB is a quorum sensing motility regulating 2 component regulon protein), as well as motA, another motility related gene.	complete CACAO 5055
ECOLI:MQSR	involved_in	GO:0044010: single-species biofilm formation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:MQSR	involved_in	GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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