GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16269004

From GONUTS
Jump to: navigation, search
Citation

Kitani, T, Okuno, S, Nakamura, Y, Tokuno, H, Takeuchi, M and Fujisawa, H (2006) Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain. J. Neurochem. 96:374-84

Abstract

Ca2+/Calmodulin-dependent protein kinase (CaM kinase) phosphatase, occurring in the cytoplasm of all tissues, dephosphorylates and thereby deactivates multifunctional CaM kinases, such as CaM kinases I, II and IV. In contrast, CaM kinase phosphatase N has been reported to occur almost exclusively in the brain and to be localized in the nucleus in the transfected COS-7 cells, as examined immunocytochemically with antibodies against the carboxyl-terminal segment of the enzyme, indicating its involvement in the deactivation of CaM kinase IV. Here, we show that the majority of the naturally occurring CaM kinase phosphatase N in the brain exists not in the intact form of the enzyme (83.4 kDa) but in a form (61.1 kDa) in which the carboxyl-terminal segment containing nuclear localization signals is deleted, and that it is present mostly in the cytoplasm but a little in the nucleus throughout the central nervous system, although occurring mostly in the nucleus in some large neurons. Strong immunostaining of the enzyme was also observed at postsynaptic density. These findings suggest that CaM kinase phosphatase N is involved in the regulation of not only CaM kinase IV but also CaM kinases II and I.

Links

PubMed Online version:10.1111/j.1471-4159.2005.03540.x

Keywords

Amino Acid Sequence; Animals; Blotting, Western; Brain/enzymology; COS Cells; Cercopithecus aethiops; Cytosol/enzymology; DNA Repair; Female; Immunohistochemistry/methods; Molecular Sequence Data; Molecular Weight; Phosphoprotein Phosphatases/genetics; Phosphoprotein Phosphatases/metabolism; Protein Phosphatase 2C; Protein Processing, Post-Translational; Rats; Rats, Wistar; Staining and Labeling; Subcellular Fractions/enzymology; Tissue Distribution

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:PPM1E

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:PPM1E

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:PPM1E

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:PPM1E

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt


RAT:PPM1E

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt


RAT:PPM1E

part_of

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt


Notes

See also

References

See Help:References for how to manage references in GONUTS.