PMID:16244109

Citation	Dauffy, J, Mouchiroud, G and Bourette, RP (2006) The interferon-inducible gene, Ifi204, is transcriptionally activated in response to M-CSF, and its expression favors macrophage differentiation in myeloid progenitor cells. J. Leukoc. Biol. 79:173-83
Abstract	The interferon-inducible (Ifi)204 gene was isolated as a macrophage-colony stimulating factor (M-CSF)-responsive gene using a gene trap approach in the myeloid interleukin-3 (IL-3)-dependent FD-Fms cell line, which differentiates in macrophages in response to M-CSF. Here, we show that Ifi204 was transcriptionally activated in response to M-CSF, and FD-Fms cells decreased their growth and committed toward a macrophage morphology; this induction was abrogated when the differentiation signal of the M-CSF receptor was blocked; the Ifi204 gene was also induced during macrophage differentiation controlled by leukemia inhibitory factor; and the Ifi204 gene is expressed in different mature monocyte/macrophage cells. Finally, we showed that enforced expression of Ifi204 strongly decreased IL-3- and M-CSF-dependent proliferation and conversely, favored macrophage differentiation of FD-Fms cells in response to M-CSF. Altogether, these results demonstrate that the Ifi204 gene is activated during macrophage development and suggest that the Ifi204 protein may act as a regulator of the balance between proliferation and differentiation. Moreover, this study suggests that other members of the Ifi family might act as regulators of hematopoiesis under the control of hemopoietic cytokines.
Links	PubMed Online version:10.1189/jlb.0205083
Keywords	Animals; Cell Differentiation/drug effects; Cell Differentiation/immunology; Cell Line; Cell Proliferation/drug effects; Gene Expression Regulation/drug effects; Gene Expression Regulation/immunology; Hematopoiesis/genetics; Hematopoiesis/immunology; Humans; Interleukin-3/immunology; Interleukin-3/metabolism; Interleukin-6/immunology; Interleukin-6/metabolism; Leukemia Inhibitory Factor; Macrophage Activation/drug effects; Macrophage Activation/genetics; Macrophage Activation/immunology; Macrophage Colony-Stimulating Factor/immunology; Macrophage Colony-Stimulating Factor/pharmacology; Macrophages/immunology; Macrophages/metabolism; Mice; Myeloid Progenitor Cells/immunology; Myeloid Progenitor Cells/metabolism; Nuclear Proteins/biosynthesis; Nuclear Proteins/genetics; Nuclear Proteins/immunology; Phosphoproteins/biosynthesis; Phosphoproteins/genetics; Phosphoproteins/immunology; Signal Transduction/drug effects; Signal Transduction/genetics; Signal Transduction/immunology; Transcription, Genetic/drug effects; Transcription, Genetic/immunology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:IFI4		GO:0043032: positive regulation of macrophage activation	ECO:0000314:		P		Figure 6C: Analysis of RT-PCR showed that enforced expression of Ifi204 favors M-CSF-dependent macrophage differentiation in in FD-Fms cells.	complete

References

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