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PMID:16199588
| Citation |
O'Flaherty, S, Coffey, A, Meaney, W, Fitzgerald, GF and Ross, RP (2005) The recombinant phage lysin LysK has a broad spectrum of lytic activity against clinically relevant staphylococci, including methicillin-resistant Staphylococcus aureus. J. Bacteriol. 187:7161-4 |
|---|---|
| Abstract |
This study concerns the cloning, characterization, and expression of the lysin (LysK) from staphylococcal phage K in Lactococcus lactis. Lactococcal lysates containing recombinant LysK were found to inhibit a range of different species of staphylococci isolated from bovine and human infection sources, including methicillin-resistant Staphylococcus aureus. LysK thus has potential as an antimicrobial for applications in the prevention and/or treatment of infections caused by staphylococci. |
| Links |
PubMed PMC1251611 Online version:10.1128/JB.187.20.7161-7164.2005 |
| Keywords |
Animals; Anti-Bacterial Agents; Bacteriolysis/genetics; Cattle; Enzymes/genetics; Enzymes/metabolism; Humans; Lactococcus lactis/genetics; Methicillin Resistance; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Staphylococcal Infections/drug therapy; Staphylococcal Infections/microbiology; Staphylococcus Phages/genetics; Staphylococcus aureus/drug effects; Staphylococcus aureus/virology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0001897: cytolysis by symbiont of host cells |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0001897: cytolysis by symbiont of host cells |
ECO:0000314: |
P |
Figure 1 lane 5 shows that extract from E.coli that are induced to express LysK creates a zone of clearing in a zymogram loaded with MRSA cells, indicating lysis. |
complete | ||||
See also
References
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