BPPGK:Q6Y7T6

Species (Taxon ID)	Staphylococcus phage K. (221915)
Gene Name(s)	No Information Provided.
Protein Name(s)	ORF30/ORF32 (ECO:0000313 with EMBL:AAO47477.2) Putative endolysin (ECO:0000313 with EMBL:AHB79986.1)
External Links
UniProt	Q6Y7T6
EMBL	AY176327 KF766114
RefSeq	YP_009041293.1
PDB	4CSH 4CT3
PDBsum	4CSH 4CT3
GeneID	19622106
Proteomes	UP000001246
GO	GO:0046872 GO:0008745 GO:0051715 GO:0009253
Gene3D	3.40.80.10
InterPro	IPR002502 IPR007921 IPR003646
Pfam	PF01510 PF05257 PF08460
SMART	SM00644 SM00287
SUPFAM	SSF55846
PROSITE	PS50911

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0008745	N-acetylmuramoyl-L-alanine amidase activity	PMID:19493008	ECO:0000314			F		Figure 1 shows the EIMS analysis of products of LysK reacting with S.aureus PG. Frame B of figure 1 shows where the enzyme must cut to form the product shown in frame A, between the NAM and the L-ala.	complete CACAO 6015
	GO:0001897	cytolysis by symbiont of host cells	PMID:19493008	ECO:0000314			P		Figure 5 shows LysK is able to lyse various Staph species.	complete CACAO 6016
	GO:0001897	cytolysis by symbiont of host cells	PMID:16199588	ECO:0000314			P		Figure 1 lane 5 shows that extract from E.coli that are induced to express LysK creates a zone of clearing in a zymogram loaded with MRSA cells, indicating lysis.	complete CACAO 6017
	GO:0008233	peptidase activity	PMID:19493008	ECO:0000315			F		Figure 3: A series of LysK deletion constructs were tested using turbidity reduction assays. Constructs lacking the SH3B domain, LysK 211 and 390 showed 10-fold less activity then wild-type LysK. SH3B domains alone, 325-495 showed no discernable activity. 10μL spots containing 10μg of LysK297, or LysK149-495 had no discernable activity in the plate lysis assay. Fusion of SH3B to the CHAP domain, LysK221-390 showed increased activity of the CHAP domain.	complete CACAO 13103
involved_in	GO:0001897	cytolysis by symbiont of host cells	PMID:19493008	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0001897	cytolysis by symbiont of host cells	PMID:16199588	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0008745	N-acetylmuramoyl-L-alanine amidase activity	PMID:19493008	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0008745	N-acetylmuramoyl-L-alanine amidase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR002502 InterPro:IPR036505	F		Seeded From UniProt	complete
involved_in	GO:0009253	peptidoglycan catabolic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR002502 InterPro:IPR036505	P		Seeded From UniProt	complete
enables	GO:0046872	metal ion binding	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0479	F		Seeded From UniProt	complete
edit table

References

See Help:References for how to manage references in GONUTS.