GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:1597420

From GONUTS
Jump to: navigation, search
Citation

Choi, SH and Greenberg, EP (1992) Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein. J. Bacteriol. 174:4064-9

Abstract

The Vibrio fischeri luminescence (lux) genes are regulated by the 250-amino-acid-residue LuxR protein and a V. fischeri metabolite termed autoinducer. The V. fischeri lux regulon consists of two divergently transcribed units. Autoinducer and LuxR activate transcription of the luxICDABE operon and autoregulate the luxR transcriptional unit. LuxR proteins with C-terminal truncations of up to 40 amino acid residues coded by plasmids with luxR 3'-deletion mutations are functional in negative autoregulation as demonstrated by using a luxR::lacZ transcriptional fusion as a luxR promoter probe in Escherichia coli. The truncated LuxR proteins showed little or no ability to activate transcription of luxICDABE, as indicated by using luminescence as a sensitive indicator of promoter strength in E. coli. Besides having no detectable activity as positive regulators of luxICDABE, LuxR proteins with C-terminal truncations of more than 40 amino acid residues had reduced or no detectable activity as negative autoregulators. The results suggest that amino acid residues in LuxR prior to no. 211 are sufficient for lux DNA binding. Residues in the region of 211 to 250 constitute a C-terminal tail that appears to be involved in activation of luxICDABE transcription either by interacting physically with the transcription initiation complex or by affecting lux DNA in the vicinity of the promoter.

Links

PubMed PMC206117

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Blotting, Western; DNA Mutational Analysis; DNA, Bacterial/metabolism; Gene Expression Regulation, Bacterial/genetics; Luminescent Measurements; Molecular Sequence Data; Operon/genetics; Plasmids/genetics; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Repressor Proteins; Trans-Activators; Transcription Factors/genetics; Transcription Factors/metabolism; Transcriptional Activation; Vibrio/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ALIFS:LUXR

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314:

P

Fig. 1: the operator and the arrows indicate the direction of transcription from the two characterized of promoters.

complete
CACAO 11415

Notes

See also

References

See Help:References for how to manage references in GONUTS.