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Choi, SH and Greenberg, EP (1992) Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein. J. Bacteriol. 174:4064-9


The Vibrio fischeri luminescence (lux) genes are regulated by the 250-amino-acid-residue LuxR protein and a V. fischeri metabolite termed autoinducer. The V. fischeri lux regulon consists of two divergently transcribed units. Autoinducer and LuxR activate transcription of the luxICDABE operon and autoregulate the luxR transcriptional unit. LuxR proteins with C-terminal truncations of up to 40 amino acid residues coded by plasmids with luxR 3'-deletion mutations are functional in negative autoregulation as demonstrated by using a luxR::lacZ transcriptional fusion as a luxR promoter probe in Escherichia coli. The truncated LuxR proteins showed little or no ability to activate transcription of luxICDABE, as indicated by using luminescence as a sensitive indicator of promoter strength in E. coli. Besides having no detectable activity as positive regulators of luxICDABE, LuxR proteins with C-terminal truncations of more than 40 amino acid residues had reduced or no detectable activity as negative autoregulators. The results suggest that amino acid residues in LuxR prior to no. 211 are sufficient for lux DNA binding. Residues in the region of 211 to 250 constitute a C-terminal tail that appears to be involved in activation of luxICDABE transcription either by interacting physically with the transcription initiation complex or by affecting lux DNA in the vicinity of the promoter.


PubMed PMC206117


Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Blotting, Western; DNA Mutational Analysis; DNA, Bacterial/metabolism; Gene Expression Regulation, Bacterial/genetics; Luminescent Measurements; Molecular Sequence Data; Operon/genetics; Plasmids/genetics; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Repressor Proteins; Trans-Activators; Transcription Factors/genetics; Transcription Factors/metabolism; Transcriptional Activation; Vibrio/genetics



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status


GO:0045893: positive regulation of transcription, DNA-templated



Fig. 1: the operator and the arrows indicate the direction of transcription from the two characterized of promoters.

CACAO 11415


See also


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