GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:1577270
| Citation |
Ruben, SM, Klement, JF, Coleman, TA, Maher, M, Chen, CH and Rosen, CA (1992) I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity. Genes Dev. 6:745-60 |
|---|---|
| Abstract |
The NF-kappa B transcription factor complex is comprised of two subunits, p50 and p65, that share significant homology to the rel oncogene. We have isolated a cDNA encoding a novel 66-kD rel-related protein, designated I-Rel. Unlike other rel-related proteins, I-Rel does not interact with DNA. I-Rel forms heterodimers with p50, however, and greatly attenuates its DNA-binding activity--an effect probably resulting from the presence of a domain inhibitory to DNA binding present within the 121 amino-terminal residues of I-Rel. In contrast, I-Rel does not associate with p65. Transfection experiments demonstrate that I-Rel suppresses NF-kappa B-induced transcription, probably through its association with p50. Expression of I-Rel mRNA is induced by mitogenic stimulation and accumulates after the appearance of p50 transcripts. Our findings suggest that p50 and I-Rel are components of a feedback pathway where expression of I-Rel may modulate indirectly the expression of genes responsive to the NF-kappa B transcription factor complex. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Blotting, Northern; DNA/metabolism; Escherichia coli/genetics; Gene Expression Regulation/genetics; Molecular Sequence Data; NF-kappa B/genetics; Oncogene Proteins v-rel; Phosphoproteins/chemistry; Phosphoproteins/genetics; Phosphoproteins/metabolism; Precipitin Tests; Protein-Tyrosine Kinases/chemistry; Protein-Tyrosine Kinases/genetics; Protein-Tyrosine Kinases/metabolism; Proto-Oncogene Proteins/chemistry; Proto-Oncogene Proteins/genetics; Retroviridae Proteins, Oncogenic/chemistry; Retroviridae Proteins, Oncogenic/genetics; Retroviridae Proteins, Oncogenic/metabolism; T-Lymphocytes; Transcription Factor RelB; Transcription Factors; Transcription, Genetic/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0003714: transcription corepressor activity |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
