PMID:15606898

Citation	Bailey, CD and Johnson, GV (2005) Tissue transglutaminase contributes to disease progression in the R6/2 Huntington's disease mouse model via aggregate-independent mechanisms. J. Neurochem. 92:83-92
Abstract	Huntington's disease (HD) is caused by an expansion of CAG repeats within the huntingtin gene and is characterized by intraneuronal mutant huntingtin protein aggregates. In order to determine the role of tissue transglutaminase (tTG) in HD aggregate formation and disease progression, we cross-bred the R6/2 HD mouse model with a tTG knockout mouse line. R6/2 mice that were tTG heterozygous knockouts (R6/2 : tTG+/-) and tTG homozygous knockouts (R6/2 : tTG-/-) showed a very similar increase in aggregate number within the striatum compared with R6/2 mice that were wild-type with respect to tTG (R6/2 : tTG+/+). Interestingly, a significant delay in the onset of motor dysfunction and death occurred in R6/2 : tTG-/- mice compared with both R6/2 : tTG+/+ and R6/2 : tTG+/- mice. As aggregate number was similarly increased in the striatum of both R6/2 : tTG+/- and R6/2 : tTG-/- mice, whereas only R6/2 : tTG-/- mice showed delayed disease progression, these data suggest that the contribution of tTG towards motor dysfunction and death in the R6/2 mouse is independent of its ability to negatively regulate aggregate formation. Moreover, the combined results from this study suggest that the formation of striatal huntingtin aggregates does not directly influence motor dysfunction or death in this HD mouse model.
Links	PubMed Online version:10.1111/j.1471-4159.2004.02839.x
Keywords	Animals; Cell Aggregation/physiology; Disease Models, Animal; Disease Progression; Female; GTP-Binding Proteins/deficiency; GTP-Binding Proteins/genetics; GTP-Binding Proteins/physiology; Huntington Disease/enzymology; Huntington Disease/genetics; Huntington Disease/pathology; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; Pregnancy; Prosencephalon/enzymology; Transglutaminases/deficiency; Transglutaminases/genetics; Transglutaminases/physiology
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:COF1	acts_upstream_of_or_within	GO:0007015: actin filament organization	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:3574399	P	occurs_in:(CL:0000333)	Seeded From UniProt	complete
MOUSE:COF1	acts_upstream_of_or_within	GO:0030010: establishment of cell polarity	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:3574399	P	occurs_in:(CL:0000333)	Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.