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PMID:1556090
| Citation |
Wu, ZL, Charles, TC, Wang, H and Nester, EW (1992) The ntrA gene of Agrobacterium tumefaciens: identification, cloning, and phenotype of a site-directed mutant. J. Bacteriol. 174:2720-3 |
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| Abstract |
A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization and C4-dicarboxylate transport but not for vir gene expression or tumorigenesis. |
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| Keywords |
Agrobacterium tumefaciens/genetics; Cloning, Molecular; DNA, Bacterial/genetics; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetic Complementation Test; Mutagenesis, Site-Directed; Sigma Factor/genetics |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006355: regulation of transcription, DNA-dependent |
ECO:0000315: |
P |
Figure 2 shows that ntrA mutant strains of A. tumefaciens could not grow on minimal media with succinate, fumarate, or malate as the only carbon source or nitrate as the only nitrogen source. This shows that ntrA is involved in the use of poor nitrogen sources. |
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