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PMID:15522242
| Citation |
Liao, Y, Lescar, J, Tam, JP and Liu, DX (2004) Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability. Biochem. Biophys. Res. Commun. 325:374-80 |
|---|---|
| Abstract |
To promote viral entry, replication, release, and spread to neighboring cells, many cytolytic animal viruses encode proteins responsible for modification of host cell membrane permeability and for formation of ion channels in host cell membranes during their life cycles. In this study, we show that the envelope (E) protein of severe acute respiratory syndrome-associated coronavirus can induce membrane permeability changes when expressed in Escherichia coli. E protein expressed in bacterial and mammalian cells under reducing conditions existed as monomers, but formed homodimer and homotrimer under non-reducing conditions. Site-directed mutagenesis studies revealed that two cysteine residues of the E protein were essential for oligomerization, leading to induction of membrane permeability. This is the first report demonstrating that a coronavirus-encoded protein could modify membrane permeability in E. coli cells. |
| Links |
PubMed Online version:10.1016/j.bbrc.2004.10.050 |
| Keywords |
Animals; Cell Membrane Permeability; Cysteine/metabolism; DNA Mutational Analysis; Escherichia coli/genetics; Escherichia coli/physiology; HeLa Cells; Humans; Mutagenesis, Site-Directed; Protein Conformation; SARS Virus/metabolism; Viral Envelope Proteins/chemistry; Viral Envelope Proteins/genetics; Viral Envelope Proteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0044662: disruption by virus of host cell membrane |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0044662: disruption by virus of host cell membrane |
ECO:0000314: |
P |
Figure 2B indicates cells that express E have increased permeability. ONPG is a compound that will, typically, only enter bacterial cells if the cell membrane becomes permeable. ONPG can be converted into a colored compound by β-Galactosidase. The O.D. from this colored compound was measured and the results indicate the E protein increases permeability of the cell membrane. |
complete | ||||
Notes
See also
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