PMID:15165250

Citation	Ramey, BE, Matthysse, AG and Fuqua, C (2004) The FNR-type transcriptional regulator SinR controls maturation of Agrobacterium tumefaciens biofilms. Mol. Microbiol. 52:1495-511
Abstract	Agrobacterium tumefaciens is a plant pathogen that persists as surface-associated populations on plants or soil particles. A genetic screen for A. tumefaciens mutants deficient for surface interactions identified a mutant that forms thin, sparsely populated biofilms, but is proficient for initial attachment. The mutant is disrupted in a gene designated sinR, encoding a member of the DNR subfamily of FNR-type transcription regulators. SinR is required for normal maturation of A. tumefaciens biofilms on both inert surfaces and plant tissues, and elevated sinR expression results in accelerated biofilm formation. Expression of sinR is increased close to 30-fold in cultures grown in oxygen-limited environments and is also induced within biofilms grown under oxic conditions. A consensus FNR box, the presumptive binding site for FNR-type proteins, is located upstream of the sinR promoter. FnrN, a second A. tumefaciens FNR-like regulator, is required for induction of sinR in oxygen-limited cultures, whereas SinR negatively influences its own expression. FnrN influences biofilm formation, but its effects are less dramatic than those of SinR. We propose a model in which a signal cascade, responsive to oxygen limitation and initiated by FnrN, activates sinR expression in response to decreased oxygen levels, and influences the formation of A. tumefaciens biofilms.
Links	PubMed Online version:10.1111/j.1365-2958.2004.04079.x
Keywords	Agrobacterium tumefaciens/genetics; Agrobacterium tumefaciens/physiology; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Biofilms; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Oxygen/metabolism; Phenotype; Plants/microbiology; Repressor Proteins/genetics; Repressor Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
AGRT5:Q7CX59		GO:0006355: regulation of transcription, DNA-dependent	ECO:0000314:		P		Figure 7 in this paper expresses the effects of protein sinR when known binding site FNR's conditions are altered. This infers that this gene has to do with DNA binding and transcription factors.	complete
AGRT5:A9CIV6		GO:0006355: regulation of transcription, DNA-dependent	ECO:0000315:		P		The function of Atu 1602 was supported by figure 3.	complete

References

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