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PMID:15030778
| Citation |
Ikawa, T, Kawamoto, H, Wright, LY and Murre, C (2004) Long-term cultured E2A-deficient hematopoietic progenitor cells are pluripotent. Immunity 20:349-60 |
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| Abstract |
E2A proteins are essential for the development of B cells beyond the progenitor cell stage. Here we have isolated E2A-deficient bone marrow-derived cells that have the ability to grow long-term in vitro and coexpress, at low levels, regulators of different hematopoietic cell lineages. When transferred into lethally irradiated hosts, E2A-deficient hematopoietic progenitor cells reconstitute the T, NK, myeloid, dendritic, and erythroid lineages but fail to develop into mature B lineage cells. Enforced expression of E47 in E2A-deficient hematopoietic progenitor cells directly activates the transcription of a subset of B lineage-specific genes, including lambda5, mb-1, and Pax5. In contrast, E47 inhibits the expression of regulators of other hematopoietic lineages, including TCF-1 and GATA-1. These observations indicate that E2A-deficient hematopoietic progenitor cells remain pluripotent after long-term culture in vitro and that E2A proteins play a critical role in B cell commitment. |
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| Keywords |
Animals; B-Lymphocytes/immunology; Basic Helix-Loop-Helix Transcription Factors; Cell Lineage; Cells, Cultured; Clone Cells; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/physiology; Gene Deletion; Gene Rearrangement, B-Lymphocyte; Hematopoiesis; Hematopoietic Stem Cells/cytology; Hematopoietic Stem Cells/immunology; Hematopoietic Stem Cells/physiology; Immunoglobulin Heavy Chains/genetics; Killer Cells, Natural/immunology; Mice; Myeloid Cells/immunology; T-Lymphocytes/immunology; TCF Transcription Factors; Time Factors; Transcription Factor 7-Like 1 Protein; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription Factors/physiology; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0002326: B cell lineage commitment |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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