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PMID:1435258
| Citation |
Takano, E, Gramajo, HC, Strauch, E, Andres, N, White, J and Bibb, MJ (1992) Transcriptional regulation of the redD transcriptional activator gene accounts for growth-phase-dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2). Mol. Microbiol. 6:2797-804 |
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| Abstract |
Transcription of redD, the activator gene required for production of the red-pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transcription of redX, a biosynthetic structural gene, and the appearance of the antibiotic in the mycelium, and coincided with the intracellular appearance of ppGpp. However, ppGpp production elicited either by nutritional shift-down of, or addition of serine hydroxamate to, exponentially growing cultures had no stimulatory effect on redD transcription. The presence of redD on a multicopy plasmid resulted in elevated levels of the redD transcript and production of redX and undecylprodigiosin during exponential growth; the normal growth-phase-dependent production of undecylprodigiosin appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins; Base Sequence; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Guanosine Tetraphosphate/metabolism; Molecular Sequence Data; Prodigiosin/analogs & derivatives; Prodigiosin/biosynthesis; Promoter Regions, Genetic; Streptomyces/genetics; Streptomyces/growth & development; Streptomyces/metabolism; Trans-Activators/genetics; Transcription, Genetic |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0017000: antibiotic biosynthetic process |
ECO:0000314: |
P |
Fig 4A. After 65h, the yield of undecylprodigiosin from M145(pIJ6014) (6.1ug/ml) was 5.5 times higher than that from M145 (1.1 ug/ml), showing the increase in antibiotic production. |
complete | ||||
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involved_in |
GO:0017000: antibiotic biosynthetic process |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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