PMID:1338970

Citation	Suzuki, T, Abe-Dohmae, S and Tanaka, R (1992) P400 protein is one of the major substrates for Ca2+/calmodulin-dependent protein kinase II in the postsynaptic density-enriched fraction isolated from rat cerebral cortex, hippocampus and cerebellum. Neurochem. Int. 20:61-7
Abstract	Concanavalin A-binding glycoprotein with 250 K M(r) found in the postsynaptic density (PSD)-enriched preparation (or synaptic cytoskeleton) from rat cerebellum was identified with P400 protein from the physicochemical properties and enrichment in the cerebellum. Proteins homologous to the cerebellar 250 K M(r) protein occurred in the PSD-enriched preparations from rat cerebral cortex and from hippocampus, although the contents in the preparations were very low. The 250 K M(r) proteins in the PSD-enriched preparations from cerebellum and from cerebrum were highly phosphorylated by Ca2+/calmodulin (CaM)-dependent protein kinase II. The protein of synaptic plasma membrane (SPM) and PSD-enriched fractions prepared from cerebral cortex were not phosphorylated by the cAMP-dependent protein kinase endogenous to the fractions, whereas the protein from cerebellum was done in SPM and PSD-enriched fractions. The facts suggest that P400 or P400-like protein is closely associated with Ca2+/CaM-dependent protein kinase II in the PSD-enriched preparations, especially in the preparation from cerebral cortex. Phosphorylation of the protein by Ca2+/CaM-dependent protein kinase II may play an important role in the postsynaptic function in both cerebellum and at least in some areas of cerebrum.
Links	PubMed
Keywords	Animals; Calcium Channels; Calcium-Calmodulin-Dependent Protein Kinases; Cell Fractionation; Cerebellum/metabolism; Cerebral Cortex/metabolism; Cytoskeleton/metabolism; Electrophoresis, Polyacrylamide Gel; Hippocampus/metabolism; Inositol 1,4,5-Trisphosphate Receptors; Membrane Glycoproteins/isolation & purification; Membrane Glycoproteins/metabolism; Molecular Weight; Organ Specificity; Peptide Mapping; Phosphoproteins/isolation & purification; Phosphoproteins/metabolism; Phosphorylation; Prosencephalon/metabolism; Protein Kinases/metabolism; Rats; Receptors, Cytoplasmic and Nuclear; Subcellular Fractions/metabolism; Substrate Specificity; Synapses/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:ITPR1	part_of	GO:0014069: postsynaptic density	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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