PMID:1321066

Citation	Boynton, S and Tully, T (1992) latheo, a new gene involved in associative learning and memory in Drosophila melanogaster, identified from P element mutagenesis. Genetics 131:655-72
Abstract	Genetic dissection of learning and memory in Drosophila has been limited by the existence of ethyl methanesulfonate (EMS)-induced mutations in only a small number of X-linked genes. To remedy this shortcoming, we have begun a P element mutagenesis to screen for autosomal mutations that disrupt associative learning and/or memory. The generation of "P-tagged" mutant alleles will expedite molecular cloning of these new genes. Here, we describe a behavior-genetic characterization of latheoP1, a recessive, hypomorphic mutation of an essential gene. latheoP1 flies perform poorly in olfactory avoidance conditioning experiments. This performance deficit could not be attributed to abnormal olfactory acuity or shock reactivity-two task-relevant "peripheral" behaviors which are used during classical conditioning. Thus, the latheoP1 mutation appears to affect learning/memory specifically. Consistent with chromosomal in situ localization of the P element insertion, deficiencies of the 49F region of the second chromosome failed to complement the behavioral effect of the latheoP1 mutation. Further complementation analyses between latheoP1 and lethal alleles, produced by excision of the latheoP1 insert or by EMS or gamma-rays, in the 49F region mapped the latheo mutation to one vital complementation group. Flies heterozygous for latheoP1 and one of two EMS lethal alleles or one lethal excision allele also show the behavioral deficits, thereby demonstrating that the behavioral and lethal phenotypes co-map to the same locus.
Links	PubMed PMC1205037
Keywords	Animals; Association Learning; Chromosome Mapping; Crosses, Genetic; DNA Transposable Elements; Drosophila melanogaster/genetics; Drosophila melanogaster/physiology; Ethyl Methanesulfonate/pharmacology; Female; Genes, Lethal; Genetic Complementation Test; Heterozygote; Homozygote; Locomotion; Male; Memory; Mutagenesis; Smell
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DROME:ABL		GO:0007611: learning or memory	ECO:0000315:		P		figure 2, flies with mutant latheo gene have poor memory, statistically significant.	complete CACAO 3437
DROME:ABL	involved_in	GO:0007611: learning or memory	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

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