PMID:12582242

Citation	Yu, Q, Qiu, R, Foland, TB, Griesen, D, Galloway, CS, Chiu, YH, Sandmeier, J, Broach, JR and Bi, X (2003) Rap1p and other transcriptional regulators can function in defining distinct domains of gene expression. Nucleic Acids Res. 31:1224-33
Abstract	Barrier elements that are able to block the propagation of transcriptional silencing in yeast are functionally similar to chromatin boundary/insulator elements in metazoans that delimit functional chromosomal domains. We show that the upstream activating sequences of many highly expressed ribosome protein genes and glycolytic genes exhibit barrier activity. Analyses of these barriers indicate that binding sites for transcriptional regulators Rap1p, Abf1p, Reb1p, Adr1p and Gcn4p may participate in barrier function. We also present evidence suggesting that Rap1p is directly involved in barrier activity, and its barrier function correlates with local changes in chromatin structure. We further demonstrate that tethering the transcriptional activation domain of Rap1p to DNA is sufficient to recapitulate barrier activity. Moreover, targeting the activation domain of Adr1p or Gcn4p also establishes a barrier to silencing. These results support the notion that transcriptional regulators could also participate in delimiting functional domains in the genome.
Links	PubMed PMC150219
Keywords	Binding Sites/genetics; Chromatin/genetics; Chromatin/metabolism; DNA-Binding Proteins/genetics; Gene Expression Regulation, Fungal; Gene Silencing; Mutation; Peptide Elongation Factor 1/genetics; Protein Kinases/genetics; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Telomere-Binding Proteins/genetics; Telomere-Binding Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:RAP1	involved_in	GO:0031936: negative regulation of chromatin silencing	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.