PMID:12435673

Citation	Tsuda, H, Yamashita, Y, Shibata, Y, Nakano, Y and Koga, T (2002) Genes involved in bacitracin resistance in Streptococcus mutans. Antimicrob. Agents Chemother. 46:3756-64
Abstract	Streptococcus mutans is resistant to bacitracin, which is a peptide antibiotic produced by certain species of Bacillus. The purpose of this study was to clarify the bacitracin resistance mechanism of S. mutans. We cloned and sequenced two S. mutans loci that are involved in bacitracin resistance. The rgp locus, which is located downstream from rmlD, contains six rgp genes (rgpA to rgpF) that are involved in rhamnose-glucose polysaccharide (RGP) synthesis in S. mutans. The inactivation of RGP synthesis in S. mutans resulted in an approximately fivefold-higher sensitivity to bacitracin relative to that observed for the wild-type strain Xc. The second bacitracin resistance locus comprised four mbr genes (mbrA, mbrB, mbrC, and mbrD) and was located immediately downstream from gtfC, which encodes the water-insoluble glucan-synthesizing enzyme. Although the bacitracin sensitivities of mutants that had defects in flanking genes were similar to that of the parental strain Xc, mutants that were defective in mbrA, mbrB, mbrC, or mbrD were about 100 to 120 times more sensitive to bacitracin than strain Xc. In addition, a mutant that was defective in all of the mbrABCD genes and rgpA was more sensitive to bacitracin than either the RGP or Mbr mutants. We conclude that RGP synthesis is related to bacitracin resistance in S. mutans and that the mbr genes modulate resistance to bacitracin via an unknown mechanism that is independent of RGP synthesis.
Links	PubMed PMC132740
Keywords	Anti-Bacterial Agents/pharmacology; Bacitracin/pharmacology; Drug Resistance, Bacterial/genetics; Genomic Library; Microbial Sensitivity Tests; Plasmids/genetics; Streptococcus mutans/drug effects; Streptococcus mutans/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
STRMG:Q8VUG9		GO:0046677: response to antibiotic	ECO:0000315:		P		Figure 1	complete CACAO 5531
STRMG:Q8VUG9		GO:0046677: response to antibiotic	ECO:0000314:		P		Figure 1: It is showing that strain XcB2, which is a mbrD mutant, is more sensitive to bacitracin.	complete CACAO 5423

References

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