PMID:12399506

Citation	Lemos, JA and Burne, RA (2002) Regulation and Physiological Significance of ClpC and ClpP in Streptococcus mutans. J. Bacteriol. 184:6357-66
Abstract	Tolerance of environmental stress, especially low pH, by Streptococcus mutans is central to the virulence of this organism. The Clp ATPases are implicated in the tolerance of, and regulation of the response to, stresses by virtue of their protein reactivation and remodeling activities and their capacity to target misfolded proteins for degradation by the ClpP peptidase. The purpose of this study was to dissect the role of selected clp genes in the stress responses of S. mutans, with a particular focus on acid tolerance and adaptation. Homologues of the clpB, clpC, clpE, clpL, clpX, and clpP genes were identified in the S. mutans genome. The expression of clpC and clpP, which were chosen as the focus of this study, was induced at low pH and at growth above 40 degrees C. Inactivation of ctsR, the first of two genes in the clpC operon, demonstrated that CtsR acts as a repressor of clp and groES-EL gene expression. Strains lacking ClpP, but not strains lacking ClpC, were impaired in their ability to grow under stress-inducing conditions, formed long chains, aggregated in culture, had reduced genetic transformation efficiencies, and had a reduced capacity to form biofilms. Comparison of two-dimensional protein gels from wild-type cells and the ctsR and clpP mutants revealed many changes in the protein expression patterns. In particular, in the clpP mutant, there was an increased production of GroESL and DnaK, suggesting that cells were stressed, probably due to the accumulation of denatured proteins.
Links	PubMed PMC151938
Keywords	Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biofilms/growth & development; Endopeptidase Clp; Gene Expression Regulation, Bacterial; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Heat-Shock Response; Hot Temperature; Hydrogen-Ion Concentration; Operon; Serine Endopeptidases/genetics; Serine Endopeptidases/metabolism; Streptococcus mutans/genetics; Streptococcus mutans/growth & development; Streptococcus mutans/physiology; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
STRMU:CLPP	involved_in	GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
STRMU:CLPP		GO:0045892: negative regulation of transcription, DNA-dependent	ECO:0000315:		P		Figure 5: Comparing CtsR-deficient and wild-type strains of S. mutans, Northern Blot analysis showed that groEL, clpE, and clpP mRNAs were present in greater amounts in the mutant strain.	complete CACAO 9147
STRMU:Q8DVD0	involved_in	GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
STRMU:Q8DVD0		GO:0045892: negative regulation of transcription, DNA-dependent	ECO:0000315:		P		Figure 5: Comparing CtsR-deficient and wild-type strains of S. mutans, Northern Blot analysis showed that groEL, clpE, and clpP mRNAs were present in greater amounts in the mutant strain.	complete CACAO 9149
STRMU:CH60		GO:0032269: negative regulation of cellular protein metabolic process	ECO:0000315:		P		Figure 5: Comparing CtsR-deficient and wild-type strains of S. mutans, Northern Blot analysis showed that groEL, clpE, and clpP mRNAs were present in greater amounts in the mutant strain.	complete CACAO 9151
STRMU:Q8DS13	involved_in	GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
STRMU:Q8DS13		GO:0045892: negative regulation of transcription, DNA-dependent	ECO:0000315:		P		Figure 5: Comparing CtsR-deficient and wild-type strains of S. mutans, Northern Blot analysis showed that groEL, clpE, and clpP mRNAs were present in greater amounts in the mutant strain.	complete CACAO 9152

References

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