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PMID:11739958
| Citation |
Loeffler, JM, Nelson, D and Fischetti, VA (2001) Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase. Science 294:2170-2 |
|---|---|
| Abstract |
Nasopharyngeal carriage is the major reservoir for Streptococcus pneumoniae in the community. Although eliminating this reservoir would greatly reduce disease occurrence, no suitable intervention has been available for this purpose. We show here that seconds after contact, a purified pneumococcal bacteriophage lytic enzyme (Pal) is able to kill 15 common serotypes of pneumococci, including highly penicillin-resistant strains. In vivo, previously colonized mice revealed undetectable pneumococcal titers 5 hours after a single enzyme treatment. Pal enzyme had little or no effect on microorganisms normally found in the human oropharynx, and Pal-resistant pneumococci could not be detected after extensive exposure to the enzyme. |
| Links |
PubMed Online version:10.1126/science.1066869 |
| Keywords |
Animals; Bacterial Capsules/physiology; Bacteriolysis; Cell Membrane/drug effects; Cell Membrane/ultrastructure; Cell Wall/drug effects; Cell Wall/ultrastructure; Colony Count, Microbial; Drug Resistance, Bacterial; Humans; Mice; Mutation; N-Acetylmuramoyl-L-alanine Amidase/metabolism; N-Acetylmuramoyl-L-alanine Amidase/pharmacology; Nasopharynx/microbiology; Random Allocation; Streptococcus/drug effects; Streptococcus/growth & development; Streptococcus Phages/enzymology; Streptococcus pneumoniae/drug effects; Streptococcus pneumoniae/growth & development; Streptococcus pneumoniae/physiology; Streptococcus pneumoniae/ultrastructure |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0044659: cytolysis by virus of host cell |
ECO:0000314: |
P |
Figure 1 shows Pal induced lysis of a large number of Streptococcus pneumoniae strains. Figure 2 (B-D) also shows lysis compared to the control cells (A). |
complete | ||||
|
involved_in |
GO:0044659: cytolysis by virus of host cell |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
Notes
See also
References
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