GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:11602748
| Citation |
Yang, H, Makeyev, EV and Bamford, DH (2001) Comparison of polymerase subunits from double-stranded RNA bacteriophages. J. Virol. 75:11088-95 |
|---|---|
| Abstract |
The family Cystoviridae comprises several bacteriophages with double-stranded RNA (dsRNA) genomes. We have previously purified the catalytic polymerase subunit (Pol) of one of the Cystoviridae members, bacteriophage phi6, and shown that the protein can catalyze RNA synthesis in vitro. In this reaction, both bacteriophage-specific and heterologous RNAs can serve as templates, but those containing 3' termini from the phi6 minus strands are favored. This provides a molecular basis for the observation that only plus strands, not minus strands, are transcribed from phi6 dsRNA segments in vivo. To test whether such a regulatory mechanism is also found in other dsRNA viruses, we purified recombinant Pol subunits from the phi6-related bacteriophages phi8 and phi13 and assayed their polymerase activities in vitro. The enzymes catalyze template-dependent RNA synthesis using both single-stranded-RNA (ssRNA) and dsRNA templates. However, they differ from each other as well as from phi6 Pol in certain biochemical properties. Notably, each polymerase demonstrates a distinct preference for ssRNAs bearing short 3'-terminal sequences from the virus-specific minus strands. This suggests that, in addition to other factors, RNA transcription in Cystoviridae is controlled by the template specificity of the polymerase subunit. |
| Links |
PubMed PMC114688 Online version:10.1128/JVI.75.22.11088-11095.2001 |
| Keywords |
Amino Acid Sequence; Bacteriophage phi 6/enzymology; Bacteriophage phi 6/genetics; Base Sequence; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/isolation & purification; DNA-Directed RNA Polymerases/metabolism; Molecular Sequence Data; Protein Subunits; RNA, Double-Stranded/genetics; RNA, Viral/biosynthesis; Recombinant Proteins/isolation & purification; Transcription, Genetic |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003968: RNA-directed RNA polymerase activity |
ECO:0000314: |
F |
Figure 3B shows purified phi6 RdRp reacts with ssRNA to make dsRNA. |
complete | ||||
|
enables |
GO:0003968: RNA-directed 5'-3' RNA polymerase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0003968: RNA-directed RNA polymerase activity |
ECO:0000314: |
F |
Figure 3B shows purified phi8 Rdrp reacts with ssRNA to synthesize dsRNA. |
complete | ||||
|
enables |
GO:0003968: RNA-directed 5'-3' RNA polymerase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0003968: RNA-directed RNA polymerase activity |
ECO:0000314: |
F |
Figure 3B shows that purified phi13 Rprd can synthesize dsRNA from ssRNA. |
complete | ||||
|
enables |
GO:0003968: RNA-directed 5'-3' RNA polymerase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
